A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity **

zu Verbundenen Objekten

Abstract: Visualization of inhibitory synapses requires protocol tailoring for different sample types and imaging techniques, and usually relies on genetic manipulation or the use of antibodies that underperform in tissue immunofluorescence. Starting from an endogenous ligand of gephyrin, a universal marker of the inhibitory synapse, we developed a short peptidic binder and dimerized it, significantly increasing affinity and selectivity. We further tailored fluorophores to the binder, yielding “Sylite”—a probe with outstanding signal‐to‐background ratio that outperforms antibodies in tissue staining with rapid and efficient penetration, mitigation of staining artifacts, and simplified handling. In super‐resolution microscopy Sylite precisely localizes the inhibitory synapse and enables nanoscale measurements. Sylite profiles inhibitory inputs and synapse sizes of excitatory and inhibitory neurons in the midbrain and combined with complimentary tracing techniques reveals the synaptic connectivity.

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch

Erschienen in
A Versatile Synthetic Affinity Probe Reveals Inhibitory Synapse Ultrastructure and Brain Connectivity ** ; day:06 ; month:05 ; year:2022 ; extent:1
Angewandte Chemie / International edition. International edition ; (06.05.2022) (gesamt 1)

Urheber
Khayenko, Vladimir
Schulte, Clemens
Reis, Sara L.
Avraham, Orly
Schietroma, Cataldo
Worschech, Rafael
Nordblom, Noah F.
Kachler, Sonja
Villmann, Carmen
Heinze, Katrin G.
Schlosser, Andreas
Schueler‐Furman, Ora
Tovote, Philip
Specht, Christian G.
Maric, Hans-Michael

DOI
10.1002/anie.202202078
URN
urn:nbn:de:101:1-2022050715035336537599
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
15.08.2025, 07:28 MESZ

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Beteiligte

  • Khayenko, Vladimir
  • Schulte, Clemens
  • Reis, Sara L.
  • Avraham, Orly
  • Schietroma, Cataldo
  • Worschech, Rafael
  • Nordblom, Noah F.
  • Kachler, Sonja
  • Villmann, Carmen
  • Heinze, Katrin G.
  • Schlosser, Andreas
  • Schueler‐Furman, Ora
  • Tovote, Philip
  • Specht, Christian G.
  • Maric, Hans-Michael

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