Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin‐1
Abstract: The interaction of human galectin‐1 with a variety of oligosaccharides, from di‐(N‐acetyllactosamine) to tetra‐saccharides (blood B type‐II antigen) has been scrutinized by using a combined approach of different NMR experiments, molecular dynamics (MD) simulations, and isothermal titration calorimetry. Ligand‐ and receptor‐based NMR experiments assisted by computational methods allowed proposing three‐dimensional structures for the different complexes, which explained the lack of enthalpy gain when increasing the chemical complexity of the glycan. Interestingly, and independently of the glycan ligand, the entropy term does not oppose the binding event, a rather unusual feature for protein‐sugar interactions. CLEANEX‐PM and relaxation dispersion experiments revealed that sugar binding affected residues far from the binding site and described significant changes in the dynamics of the protein. In particular, motions in the microsecond‐millisecond timescale in residues at the protein dimer interface were identified in the presence of high affinity ligands. The dynamic process was further explored by extensive MD simulations, which provided additional support for the existence of allostery in glycan recognition by human galectin‐1.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Unravelling the Time Scale of Conformational Plasticity and Allostery in Glycan Recognition by Human Galectin‐1 ; volume:26 ; number:67 ; year:2020 ; pages:15643-15653 ; extent:11
Chemistry - a European journal ; 26, Heft 67 (2020), 15643-15653 (gesamt 11)
- Creator
-
Bertuzzi, Sara
Gimeno, Ana
Núñez‐Franco, Reyes
Bernardo‐Seisdedos, Ganeko
Delgado, Sandra
Jiménez‐Osés, Gonzalo
Millet, Oscar
Jiménez‐Barbero, Jesús
Ardá, Ana
- DOI
-
10.1002/chem.202003212
- URN
-
urn:nbn:de:101:1-2022060416293897109569
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:24 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Bertuzzi, Sara
- Gimeno, Ana
- Núñez‐Franco, Reyes
- Bernardo‐Seisdedos, Ganeko
- Delgado, Sandra
- Jiménez‐Osés, Gonzalo
- Millet, Oscar
- Jiménez‐Barbero, Jesús
- Ardá, Ana
Other Objects (12)
Conformational Heterogeneity of the HIV Envelope Glycan Shield
Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis
Conformational Analysis and Application in Diagnostic of Glycosylphosphatidylinositol Glycan Derivatives
Unravelling conformational flexibility and chirality in molecules using microwave spectroscopy
Caenorhabditis elegans N-glycan Core beta-galactoside Confers Sensitivity towards Nematotoxic Fungal Galectin CGL2
Role of galectin-glycan circuits in reproduction: from healthy pregnancy to preterm birth (PTB)
Conformational Change of Amyloid-β 40 in Association with Binding to GM1-Glycan Cluster
Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3
Cell-based glycan arrays for probing glycan–glycan binding protein interactions
The GET insertase exhibits conformational plasticity and induces membrane thinning
Conformational Plasticity and Binding Affinity Enhancement Controlled by Linker Derivatization in Macrocycles
Crystal structure analysis of anti-V2 mAb 2158 suggests a conformational epitope involving an N-linked glycan
Conformational Heterogeneity of the HIV Envelope Glycan Shield
Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis
Conformational Analysis and Application in Diagnostic of Glycosylphosphatidylinositol Glycan Derivatives
Unravelling conformational flexibility and chirality in molecules using microwave spectroscopy
Caenorhabditis elegans N-glycan Core beta-galactoside Confers Sensitivity towards Nematotoxic Fungal Galectin CGL2
Role of galectin-glycan circuits in reproduction: from healthy pregnancy to preterm birth (PTB)
Conformational Change of Amyloid-β 40 in Association with Binding to GM1-Glycan Cluster
Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3
Cell-based glycan arrays for probing glycan–glycan binding protein interactions
The GET insertase exhibits conformational plasticity and induces membrane thinning
Conformational Plasticity and Binding Affinity Enhancement Controlled by Linker Derivatization in Macrocycles
Crystal structure analysis of anti-V2 mAb 2158 suggests a conformational epitope involving an N-linked glycan
Conformational Heterogeneity of the HIV Envelope Glycan Shield
Conformational alteration in glycan induces phospholipase Cβ1 activation and angiogenesis
Conformational Analysis and Application in Diagnostic of Glycosylphosphatidylinositol Glycan Derivatives
Unravelling conformational flexibility and chirality in molecules using microwave spectroscopy
Caenorhabditis elegans N-glycan Core beta-galactoside Confers Sensitivity towards Nematotoxic Fungal Galectin CGL2
Role of galectin-glycan circuits in reproduction: from healthy pregnancy to preterm birth (PTB)
Conformational Change of Amyloid-β 40 in Association with Binding to GM1-Glycan Cluster
Twist1 regulates macrophage plasticity to promote renal fibrosis through galectin-3
Cell-based glycan arrays for probing glycan–glycan binding protein interactions
The GET insertase exhibits conformational plasticity and induces membrane thinning
Conformational Plasticity and Binding Affinity Enhancement Controlled by Linker Derivatization in Macrocycles