Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis
Abstract: Long noncoding RNA (lncRNA) highly upregulated in liver cancer (HULC) has been reported to be implicated in chemoresistance. However, the potential mechanism of HULC in paclitaxel (PTX)-resistant ovarian cancer (OC) remains undefined. The expression of RNAs and proteins was measured by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) and Western blot assay. The PTX resistance and apoptotic rate were assessed via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry, respectively. Furthermore, the interaction between miR-137 and HULC or integrin beta-8 (ITGB8) was predicted by miRcode and starBase v2.0 and then verified by dual luciferase reporter and RNA pull-down assays. In addition, the xenograft mice model was established to explore the effects of HULC in vivo. HULC was significantly upregulated and miR-137 was downregulated in PTX-resistant OC tissues and cells. Also, the HULC depletion suppressed tumor growth and PTX resistance in PTX-treated mice. miR-137 was verified as a target of HULC and directly targeted ITGB8. And HULC knockdown downregulated ITGB8 expression by targeting miR-137. miR-137 inhibitor or ITGB8 overexpression mitigated the suppressive impacts of HULC knockdown on PTX resistance. Collectively, HULC modulated ITGB8 expression to promote PTX resistance of OC by sponging miR-137.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis ; volume:16 ; number:1 ; year:2021 ; pages:667-681 ; extent:15
Open life sciences ; 16, Heft 1 (2021), 667-681 (gesamt 15)
- Urheber
-
Huang, Bo
Wei, Min
Hong, Li
- DOI
-
10.1515/biol-2021-0058
- URN
-
urn:nbn:de:101:1-2022091915215597891766
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:36 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Huang, Bo
- Wei, Min
- Hong, Li
Ähnliche Objekte (12)
Long non-coding RNA: dancing on immune stage
A NEW SPATIAL AND TEMPORAL FUSION MODEL
LncRNA MEG8 promotes NSCLC progression by modulating the miR-15a-5p-miR-15b-5p/PSAT1 axis
Searching for traveling wave solutions of nonlinear evolution equations in mathematical physics
Using statistical models to depict the response of multi-time scales drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5 models
Using statistical models to depict the response of multi-timescale drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5-Models
LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
New Clinical Application of Chaihu Decoction
Metabolically targeting immunosuppression and immunoescape for future cancer immunotherapy: a narrative review
Active microscope stabilization in three dimensions using image correlation
Long non-coding RNA: dancing on immune stage
A NEW SPATIAL AND TEMPORAL FUSION MODEL
LncRNA MEG8 promotes NSCLC progression by modulating the miR-15a-5p-miR-15b-5p/PSAT1 axis
Searching for traveling wave solutions of nonlinear evolution equations in mathematical physics
Using statistical models to depict the response of multi-time scales drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5 models
Using statistical models to depict the response of multi-timescale drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5-Models
LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
New Clinical Application of Chaihu Decoction
Metabolically targeting immunosuppression and immunoescape for future cancer immunotherapy: a narrative review
Active microscope stabilization in three dimensions using image correlation
Long non-coding RNA: dancing on immune stage
A NEW SPATIAL AND TEMPORAL FUSION MODEL
LncRNA MEG8 promotes NSCLC progression by modulating the miR-15a-5p-miR-15b-5p/PSAT1 axis
Searching for traveling wave solutions of nonlinear evolution equations in mathematical physics
Using statistical models to depict the response of multi-time scales drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5 models
Using statistical models to depict the response of multi-timescale drought to forest cover change across climate zones
Seasonal prediction skill of East Asian summer monsoon in CMIP5-Models
LncRNA PVT1 promotes cervical cancer progression by sponging miR-503 to upregulate ARL2 expression
New Clinical Application of Chaihu Decoction
Metabolically targeting immunosuppression and immunoescape for future cancer immunotherapy: a narrative review