Incorporation of reporter genes into mitochondrial DNA in budding yeast
Abstract: Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, Saccharomyces cerevisiae. Mitochondrially encoded reporter constructs can be used to interrogate various aspects of mitochondrial gene expression. The power of this technique is exemplified by a mitochondrially encoded nanoluciferase, which allows to monitor levels of mitochondrial translation under a variety of growth conditions
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
ISSN: 2666-1667
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2024
- Urheber
- DOI
-
10.1016/j.xpro.2022.101359
- URN
-
urn:nbn:de:bsz:25-freidok-2456661
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
- 14.08.2025, 10:50 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Rzepka, Magdalena
- Suhm, Tamara
- Ott, Martin
- Universität
Entstanden
- 2024
Ähnliche Objekte (12)
Modeling, simulation, and control of budding yeast cultures
Characterization of the budding yeast microtubule polymerase Stu2
Phosphoproteome dynamics during mitotic exit in budding yeast
Conservation of the COP9/signalosome in budding yeast
Engineering a synthetic p53-Mdm2 network in budding yeast
Factors driving metabolic diversity in the budding yeast subphylum
Parasite epigenetics and immune evasion: lessons from budding yeast
Bub2 regulation of cytokinesis and septation in budding yeast
In vivo characterisation of fluorescent proteins in budding yeast
Dynamics of cortical actin in budding yeast Saccharomyces cerevisiae
Systematic identification of cell size regulators in budding yeast
The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast
Modeling, simulation, and control of budding yeast cultures
Characterization of the budding yeast microtubule polymerase Stu2
Phosphoproteome dynamics during mitotic exit in budding yeast
Conservation of the COP9/signalosome in budding yeast
Engineering a synthetic p53-Mdm2 network in budding yeast
Factors driving metabolic diversity in the budding yeast subphylum
Parasite epigenetics and immune evasion: lessons from budding yeast
Bub2 regulation of cytokinesis and septation in budding yeast
In vivo characterisation of fluorescent proteins in budding yeast
Dynamics of cortical actin in budding yeast Saccharomyces cerevisiae
Systematic identification of cell size regulators in budding yeast
The nascent polypeptide-associated complex subunit Egd1 is required for efficient selective mitochondrial degradation in budding yeast
Modeling, simulation, and control of budding yeast cultures
Characterization of the budding yeast microtubule polymerase Stu2
Phosphoproteome dynamics during mitotic exit in budding yeast
Conservation of the COP9/signalosome in budding yeast
Engineering a synthetic p53-Mdm2 network in budding yeast
Factors driving metabolic diversity in the budding yeast subphylum
Parasite epigenetics and immune evasion: lessons from budding yeast
Bub2 regulation of cytokinesis and septation in budding yeast
In vivo characterisation of fluorescent proteins in budding yeast
Dynamics of cortical actin in budding yeast Saccharomyces cerevisiae
Systematic identification of cell size regulators in budding yeast