GLASSgo in Galaxy: high-throughput, reproducible and easy-to-integrate prediction of sRNA homologs

Abstract: Motivation
The correct prediction of bacterial sRNA homologs is a prerequisite for many downstream analyses based on comparative genomics, but it is frequently challenging due to the short length and distinct heterogeneity of such homologs. GLobal Automatic Small RNA Search go (GLASSGO) is an efficient tool for the prediction of sRNA homologs from a single input query. To make the algorithm available to a broader community, we offer a Docker container along with a free-access web service. For non-computer scientists, the web service provides a user-friendly interface. However, capabilities were lacking so far for batch processing, version control and direct interaction with compatible software applications as a workflow management system can provide.

Results
Here, we present GLASSGO 1.5.2, an updated version that is fully incorporated into the workflow management system GALAXY. The improved version contains a new feature for extracting the upstream regions, allowing the search for conserved promoter elements. Additionally, it supports the use of accession numbers instead of the outdated GI numbers, which widens the applicability of the tool.

Availability and implementation
GLASSGO is available at https://github.com/lotts/GLASSgo/ under the MIT license and is accompanied by instruction and application data. Furthermore, it can be installed into any GALAXY instance using the GALAXY ToolShed

Location
Deutsche Nationalbibliothek Frankfurt am Main
Extent
Online-Ressource
Language
Englisch
Notes
Bioinformatics. - 36, 15 (2020) , 4357-4359, ISSN: 1460-2059

Event
Veröffentlichung
(where)
Freiburg
(who)
Universität
(when)
2022
Creator

DOI
10.1093/bioinformatics/btaa556
URN
urn:nbn:de:bsz:25-freidok-2303868
Rights
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Last update
15.08.2025, 7:22 AM CEST

Data provider

This object is provided by:
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Associated

Time of origin

  • 2022

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