CXCR4 peptide-based fluorescence endoscopy in a mouse model of Barrett’s esophagus

Abstract: Background
Near-infrared (NIR) fluorescence imaging has been emerging as a promising strategy to overcome the high number of early esophageal adenocarcinomas missed by white light endoscopy and random biopsy collection. We performed a preclinical assessment of fluorescence imaging and endoscopy using a novel CXCR4-targeted fluorescent peptide ligand in the L2-IL1B mouse model of Barrett’s esophagus.

Methods
Six L2-IL1B mice with advanced stage of disease (12–16 months old) were injected with the CXCR4-targeted, Sulfo-Cy5-labeled peptide (MK007), and ex vivo wide-field imaging of the whole stomach was performed 4 h after injection. Before ex vivo imaging, fluorescence endoscopy was performed in three L2-IL1B mice (12–14 months old) by a novel imaging system with two L2-IL1B mice used as negative controls.

Results
Ex vivo imaging and endoscopy in L2-IL1B mice showed that the CXCR4-targeted MK007 accumulated mostly in the dysplastic lesions with a mean target-to-background ratio > 2. The detection of the Sulfo-Cy5 signal in dysplastic lesions and its co-localization with CXCR4 stained cells by confocal microscopy further confirmed the imaging results.

Conclusions
This preliminary preclinical study shows that CXCR4-targeted fluorescence endoscopy using MK007 can detect dysplastic lesions in a mouse model of Barrett’s esophagus. Further investigations are needed to assess its use in the clinical setting

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: EJNMMI Research. - 12 (2022) , 2, ISSN: 2191-219X

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2022
• Creator: Marcazzan, Sabrina ; Carvalho, Marcos J. Braz ; Konrad, Matthias ; Strangmann, Julia ; Tenditnaya, Anna ; Baumeister, Theresa ; Schmid, Roland M. ; Wester, Hans-Jürgen ; Ntziachristos, Vasilis ; Gorpas, Dimitris ; Wang, Timothy C. ; Schottelius, Margret ; Quante, Michael

Further information

• DOI: 10.1186/s13550-021-00875-7
• URN: urn:nbn:de:bsz:25-freidok-2258917
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:31 AM CEST