Low-dose ionizing radiation effects on differentiation of HL-60 cells
Abstract: The biological effects of low-dose radiation have attracted attention, but data are currently insufficient to fully understand the beneficial role of the phenomenon. In the present study, we have investigated the effects of low doses of gamma-irradiation alone and in combination with all-trans-retinoic acid (RA) on proliferation, apoptosis and differentiation of the human promyelocytic leukemia HL-60 cells. Changes in cell behavior and protein expression were determined with the use of light and fluorescent microscopy, immunocytochemical and Western blot analysis. Low-dose irradiation with 1–100 cGy caused a dose-dependent inhibition of HL-60 cell proliferation, and induced apoptosis and differentiation to granulocytes with an increase in the number of CD15-positive cells. Pre-irradiation with 1–100 cGy for 24 h before treatment with RA promoted apoptosis but did not impair RA-induced differentiation. Both processes were associated with a decrease in the expression of the proliferating cell nuclear antigen (PCNA), BCL-2, c-MYC, and changes in both cytosolic and nuclear levels of protein tyrosine-phosphorylation as well as protein kinase C alpha or beta isoforms. These results demonstrate the beneficial role of low-dose irradiation in modulating leukemia cell proliferation, differentiation and apoptosis.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Low-dose ionizing radiation effects on differentiation of HL-60 cells ; volume:5 ; number:5 ; year:2010 ; pages:600-612 ; extent:13
Open life sciences ; 5, Heft 5 (2010), 600-612 (gesamt 13)
- Urheber
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Savickiene, Jurate
Treigyte, Grazina
Aleksandraviciene, Ceslava
Navakauskiene, Ruta
- DOI
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10.2478/s11535-010-0085-2
- URN
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urn:nbn:de:101:1-2409201902473.256514555258
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:24 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Savickiene, Jurate
- Treigyte, Grazina
- Aleksandraviciene, Ceslava
- Navakauskiene, Ruta
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