Long-term exposure to “low-dose” bisphenol A decreases mitochondrial DNA copy number, and accelerates telomere shortening in human CD8 + T cells
Abstract: Exposure to the endocrine disruptor bisphenol A (BPA) has been linked with immune disorders and increased tumour risk. Our previous work in activated human peripheral blood mononuclear cells demonstrated that exposure to “low-dose” BPA diminished telomerase activity via an ER/GPR30-ERK signalling pathway. Leukocyte telomerase activity and telomere maintenance are crucial for normal immune function and homeostasis. We thus here further studied the effects of BPA on human T cell subpopulations. Exposure to 0.3–3 nM BPA, i. e. at doses in the realm of human exposure, notably reduced telomerase activity in activated CD8 + T but not CD4 + T cells in a non-monotonic response pattern as determined by the TRAP-ELISA assay. Under long-term BPA exposure, significant telomere length shortening, reduction in mitochondrial DNA copy number, cell proliferation and IFN-γ as well as hTERT protein suppression could be observed in CD8 + lymphocytes, as analysed by qRT-PCR, flow cytometry and western blot analysis. This study extends our previous in vitro findings that “low-dose” BPA has potential negative effects on healthy human cytotoxic T cell response. These results might merit some special attention to further investigate chronic BPA exposure in the context of adaptive immune response dysfunction and early onset of cancer in man
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Scientific reports. - 10, 1 (2020) , 15786, ISSN: 2045-2322
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2020
- Creator
- DOI
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10.1038/s41598-020-72546-x
- URN
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urn:nbn:de:bsz:25-freidok-1675997
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:49 AM CEST
Data provider
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Associated
- Tran, Hoai Thi Thu
- Herz, Corinna
- Lamy, Evelyn
- Universität
Time of origin
- 2020
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Long-term exposure to "low-dose" bisphenol A decreases mitochondrial DNA copy number, and accelerates telomere shortening in human CD8 + T cells
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Low-dose levels of bisphenol A inhibit telomerase via ER/GPR30-ERK signalling, impair DNA integrity and reduce cell proliferation in primary PBMC
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Long-term exposure to "low-dose" bisphenol A decreases mitochondrial DNA copy number, and accelerates telomere shortening in human CD8 + T cells
Preclinical evaluation of isothiocyanates for tumour preventive and therapeutic application
MTBITC-induced apoptosis and cell cycle arrest of human hepatoma (HepG2) cells : a link between p53 and human telomerase?
Development of an inflammation-triggered in vitro “leaky gut” model using Caco-2/HT29-MTX-E12 combined with macrophage-like THP-1 cells or primary human-derived macrophages
BC‐11 is a covalent TMPRSS2 fragment inhibitor that impedes SARS‐CoV‐2 host cell entry
Low-dose levels of bisphenol A inhibit telomerase via ER/GPR30-ERK signalling, impair DNA integrity and reduce cell proliferation in primary PBMC
Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice
Short-term dietary intervention with cooked but not raw brassica leafy vegetables increases telomerase activity in CD8+ lymphocytes in a randomized human trial
Evaluation of an Aqueous extract from Horseradish root (Armoracia rusticana radix) against Lipopolysaccharide-induced cellular inflammation reaction
Short-Term Dietary Intervention with Cooked but Not Raw Brassica Leafy Vegetables Increases Telomerase Activity in CD8+ Lymphocytes in a Randomized Human Trial
Development of an inflammation-triggered in vitro "leaky gut" model using Caco-2/HT29-MTX-E12 combined with macrophage-like THP-1 cells or primary human-derived macrophages
BC‐11 is a covalent TMPRSS2 fragment inhibitor that impedes SARS‐CoV‐2 host cell entry
Long-term exposure to "low-dose" bisphenol A decreases mitochondrial DNA copy number, and accelerates telomere shortening in human CD8 + T cells
Preclinical evaluation of isothiocyanates for tumour preventive and therapeutic application
MTBITC-induced apoptosis and cell cycle arrest of human hepatoma (HepG2) cells : a link between p53 and human telomerase?
Development of an inflammation-triggered in vitro “leaky gut” model using Caco-2/HT29-MTX-E12 combined with macrophage-like THP-1 cells or primary human-derived macrophages
BC‐11 is a covalent TMPRSS2 fragment inhibitor that impedes SARS‐CoV‐2 host cell entry
Low-dose levels of bisphenol A inhibit telomerase via ER/GPR30-ERK signalling, impair DNA integrity and reduce cell proliferation in primary PBMC
Mdc1 deletion accelerates telomere shortening, chromosomal instability, and premature aging in telomerase deficient mice
Short-term dietary intervention with cooked but not raw brassica leafy vegetables increases telomerase activity in CD8+ lymphocytes in a randomized human trial
Evaluation of an Aqueous extract from Horseradish root (Armoracia rusticana radix) against Lipopolysaccharide-induced cellular inflammation reaction
Short-Term Dietary Intervention with Cooked but Not Raw Brassica Leafy Vegetables Increases Telomerase Activity in CD8+ Lymphocytes in a Randomized Human Trial
Development of an inflammation-triggered in vitro "leaky gut" model using Caco-2/HT29-MTX-E12 combined with macrophage-like THP-1 cells or primary human-derived macrophages