Heat-shock protein 90 controls the expression of cell-cycle genes by stabilizing metazoan-specific host-cell factor HCFC1

Abstract: Molecular chaperones such as heat-shock proteins (HSPs) help in protein folding. Their function in the cytosol has been well studied. Notably, chaperones are also present in the nucleus, a compartment where proteins enter after completing de novo folding in the cytosol, and this raises an important question about chaperone function in the nucleus. We performed a systematic analysis of the nuclear pool of heat-shock protein 90. Three orthogonal and independent analyses led us to the core functional interactome of HSP90. Computational and biochemical analyses identify host cell factor C1 (HCFC1) as a transcriptional regulator that depends on HSP90 for its stability. HSP90 was required to maintain the expression of HCFC1-targeted cell-cycle genes. The regulatory nexus between HSP90 and the HCFC1 module identified in this study sheds light on the relevance of chaperones in the transcription of cell-cycle genes. Our study also suggests a therapeutic avenue of combining chaperone and transcription inhibitors for cancer treatment

Objekt anzeigen

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: Cell reports. - 29, 6 (2019) , 1645-1659.e9, ISSN: 2211-1247

Klassifikation und Themen

• Klassifikation: Biowissenschaften, Biologie
• Schlagwort: Krebs ; Chromatin ; Hitzeschock-Proteine

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2019
• Urheber: Antonova, Aneliya ; Hummel, Barbara ; Khavaran, Ashkan ; Redhaber, Desiree Melanie ; Aprile-Garcia, Fernando ; Rawat, Prashant ; Gundel, Kathrin ; Schneck, Megan ; Hansen, Erik C. ; Mitschke, Jan ; Mittler, Gerhard ; Miething, Cornelius ; Sawarkar, Ritwick

Weitere Informationen

• DOI: 10.1016/j.celrep.2019.09.084
• URN: urn:nbn:de:bsz:25-freidok-1513121
• Rechteinformation: Der Zugriff auf das Objekt ist unbeschränkt möglich.