A comparative analysis of microglial inducible Cre lines
Abstract: Cre/loxP technology has revolutionized genetic studies and allowed for spatial and temporal control of gene expression in specific cell types. Microglial biology has particularly benefited because microglia historically have been difficult to transduce with virus or electroporation methods for gene delivery. Here, we investigate five of the most widely available microglial inducible Cre lines. We demonstrate varying degrees of recombination efficiency, cell-type specificity, and spontaneous recombination, depending on the Cre line and inter-loxP distance. We also establish best practice guidelines and protocols to measure recombination efficiency, particularly in microglia. There is increasing evidence that microglia are key regulators of neural circuits and major drivers of a broad range of neurological diseases. Reliable manipulation of their function in vivo is of utmost importance. Identifying caveats and benefits of all tools and implementing the most rigorous protocols are crucial to the growth of the field and the development of microglia-based therapeutics
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Cell reports. - 42, 9 (2023) , 113031, ISSN: 2211-1247
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
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Faust, Travis E.
Feinberg, Philip A.
O’Connor, Ciara
Kawaguchi, Riki
Chan, Andrew
Strasburger, Hayley
Frosch, Maximilian
Boyle, Margaret A.
Masuda, Takahiro
Amann, Lukas
Knobeloch, Klaus-Peter
Prinz, Marco
Schaefer, Anne
Schafer, Dorothy P.
- DOI
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10.1016/j.celrep.2023.113031
- URN
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urn:nbn:de:bsz:25-freidok-2389423
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 10:51 MESZ
Datenpartner
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Beteiligte
- Faust, Travis E.
- Feinberg, Philip A.
- O’Connor, Ciara
- Kawaguchi, Riki
- Chan, Andrew
- Strasburger, Hayley
- Frosch, Maximilian
- Boyle, Margaret A.
- Masuda, Takahiro
- Amann, Lukas
- Knobeloch, Klaus-Peter
- Prinz, Marco
- Schaefer, Anne
- Schafer, Dorothy P.
- Universität
Entstanden
- 2023
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