Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

to related objects

Abstract: The interaction in multisubunit non‐ribosomal peptide synthetases (NRPSs) is mediated by docking domains that ensure the correct subunit‐to‐subunit interaction. We introduced natural docking domains into the three‐module xefoampeptide synthetase (XfpS) to create two to three artificial NRPS XfpS subunits. The enzymatic performance of the split biosynthesis was measured by absolute quantification of the products by HPLC‐ESI‐MS. The connecting role of the docking domains was probed by deleting integral parts of them. The peptide production data was compared to soluble protein amounts of the NRPS using SDS‐PAGE. Reduced peptide synthesis was not a result of reduced soluble NRPS concentration but a consequence of the deletion of vital docking domain parts. Splitting the xefoampeptide biosynthesis polypeptide by introducing docking domains was feasible and resulted in higher amounts of product in one of the two tested split‐module cases compared to the full‐length wild‐type enzyme.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains ; volume:59 ; number:32 ; year:2020 ; pages:13463-13467 ; extent:5
Angewandte Chemie / International edition. International edition ; 59, Heft 32 (2020), 13463-13467 (gesamt 5)

Creator: Kegler, Carsten
Bode, Helge Björn

DOI: 10.1002/anie.201915989

URN: urn:nbn:de:101:1-2022061112531203802850

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:36 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Other Objects (12)

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Engineering and characterisation of non-ribosomal peptide synthetases

Engineering and characterisation of non-ribosomal peptide synthetases

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Guidelines for optimizing type S non-ribosomal peptide synthetases

Guidelines for optimizing type S non-ribosomal peptide synthetases

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Engineering and characterisation of non-ribosomal peptide synthetases

Engineering and characterisation of non-ribosomal peptide synthetases

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Guidelines for optimizing type S non-ribosomal peptide synthetases

Guidelines for optimizing type S non-ribosomal peptide synthetases

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial Splitting of a Non‐Ribosomal Peptide Synthetase by Inserting Natural Docking Domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Artificial splitting of a non‐ribosomal peptide synthetase by inserting natural docking domains

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Non-ribosomal peptide synthetase docking domains : structure, function and engineering strategies

Engineering and characterisation of non-ribosomal peptide synthetases

Engineering and characterisation of non-ribosomal peptide synthetases

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Non-ribosomal peptide synthetase engineering focusing on the condensation domain and the condensation/adenylation domain interface

Guidelines for optimizing type S non-ribosomal peptide synthetases

Guidelines for optimizing type S non-ribosomal peptide synthetases

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic Zippers as an Enabling Tool for Engineering of Non‐Ribosomal Peptide Synthetases **

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Synthetic zippers as an enabling tool for engineering of non-ribosomal peptide synthetases

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Type S Non‐Ribosomal Peptide Synthetases for the Rapid Generation of Tailormade Peptide Libraries **

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Nonribosomal Peptides Produced by Minimal and Engineered Synthetases with Terminal Reductase Domains

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.