Unique microglia expression profile in developing white matter

Abstract: Objective: Recently we demonstrated that amoeboid microglia in white matter regions are essential for proper oligodendrocyte
homeostasis and myelinogenesis in the first postnatal week. Amoeboid microglia in the mouse corpus
callosum change their activation profile within few days after postnatal day (P)7 with microglia of the cerebellum
showing similar features. Here we expanded our previous transcriptional analysis and performed detailed bulk RNA
sequencing of microglia from corpus callosum, cortex and cerebellum at P7, P10 and P42. The goal of this study was
to identify a specific gene profile for both, white matter and grey matter microglia during development.

Results: Microglia in white matter regions display unique characteristics in the first postnatal week of murine life. In
both the corpus callosum and cerebellum microglia show amoeboid morphology and a similar transcription profile
during development including high expression of genes related to priming of microglia, phagocytosis and migration
at P7; characteristics which are already lost at P10. Together these data verify our previous transcriptional data
obtained by microarray analysis and enable a more complete view into white matter and grey matter microglia at
different developmental stages

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch
• Notes: issn: 1756-0500

Classification and Topics

• Keyword: Substantia alba ; Substantia grisea

Contributors, Places and Time

• Event: Veröffentlichung
• (where): Freiburg
• (who): Universität
• (when): 2019
• Creator: Staszewski, Ori ; Hagemeyer, Nora

Further information

• DOI: 10.1186/s13104-019-4410-1
• URN: urn:nbn:de:bsz:25-freidok-1501471
• Rights: Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 25.03.2025, 1:53 PM CET