Boron‐Containing Probes for Non‐optical High‐Resolution Imaging of Biological Samples
Abstract: Boron has been employed in materials science as a marker for imaging specific structures by electron energy loss spectroscopy (EELS) or secondary ion mass spectrometry (SIMS). It has a strong potential in biological analyses as well; however, the specific coupling of a sufficient number of boron atoms to a biological structure has proven challenging. Herein, we synthesize tags containing closo‐1,2‐dicarbadodecaborane, coupled to soluble peptides, which were integrated in specific proteins by click chemistry in mammalian cells and were also coupled to nanobodies for use in immunocytochemistry experiments. The tags were fully functional in biological samples, as demonstrated by nanoSIMS imaging of cell cultures. The boron signal revealed the protein of interest, while other SIMS channels were used for imaging different positive ions, such as the cellular metal ions. This allows, for the first time, the simultaneous imaging of such ions with a protein of interest and will enable new biological applications in the SIMS field.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Boron‐Containing Probes for Non‐optical High‐Resolution Imaging of Biological Samples ; volume:131 ; number:11 ; year:2019 ; pages:3476-3481 ; extent:6
Angewandte Chemie ; 131, Heft 11 (2019), 3476-3481 (gesamt 6)
- Creator
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Kabatas, Selda
Agüi‐Gonzalez, Paola
Saal, Kim‐Ann
Jähne, Sebastian
Opazo, Felipe
Rizzoli, Silvio O.
Phan, Nhu T. N.
- DOI
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10.1002/ange.201812032
- URN
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urn:nbn:de:101:1-2022081409340499101237
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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03.01.2025, 1:32 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kabatas, Selda
- Agüi‐Gonzalez, Paola
- Saal, Kim‐Ann
- Jähne, Sebastian
- Opazo, Felipe
- Rizzoli, Silvio O.
- Phan, Nhu T. N.
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π-Conjugated Polycycle Containing Boron
Bor- und borlegierte Stähle : = Boron and boron containing steels
Boron‐Nitrogen‐Containing Benzene Valence Isomers
Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes
Development and investigation of probes for high-resolution colocalization microscopy
High-resolution tip-enhanced Raman scattering probes sub-molecular density changes
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Glaser‐Hay‐Coupled Random Copolymers Containing Boron Difluoride Formazanate Dyes
mRNA transcript quantification in archival samples using multiplexed, color-coded probes
Theoretical Investigations on Inorganic, Boron-containing Biradicals with a unique Structure
Boron‐Containing Probes for Non‐optical High‐Resolution Imaging of Biological Samples
π-Conjugated Polycycle Containing Boron
Bor- und borlegierte Stähle : = Boron and boron containing steels
Boron‐Nitrogen‐Containing Benzene Valence Isomers
Fabrication and characterization of boron-doped nanocrystalline diamond-coated MEMS probes
Development and investigation of probes for high-resolution colocalization microscopy
High-resolution tip-enhanced Raman scattering probes sub-molecular density changes
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Glaser‐Hay‐Coupled Random Copolymers Containing Boron Difluoride Formazanate Dyes
mRNA transcript quantification in archival samples using multiplexed, color-coded probes