Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles

Abstract: Interactions between membranes and biomolecular condensates can give rise to complex phenomena such as wetting transitions, mutual remodeling, and endocytosis. In this study, light‐triggered manipulation of condensate engulfment is demonstrated using giant vesicles containing photoswitchable lipids. UV irradiation increases the membrane area, which can be stored in nanotubes. When in contact with a condensate droplet, the UV light triggers rapid condensate endocytosis, which can be reverted by blue light. The affinity of the protein‐rich condensates to the membrane and the reversibility of the engulfment processes is quantified from confocal microscopy images. The degree of photo‐induced engulfment, whether partial or complete, depends on the vesicle excess area and the relative sizes of vesicles and condensates. Theoretical estimates suggest that utilizing the light‐induced excess area to increase the vesicle‐condensate adhesion interface is energetically more favorable than the energy gain from folding the membrane into invaginations and tubes. The overall findings demonstrate that membrane‐condensate interactions can be easily and quickly modulated via light, providing a versatile system for building platforms to control cellular events and design intelligent drug delivery systems for cell repair.

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• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Photoswitchable Endocytosis of Biomolecular Condensates in Giant Vesicles ; day:06 ; month:04 ; year:2024 ; extent:11 ; Advanced science ; (06.04.2024) (gesamt 11)

Beteiligte, Orts- und Zeitangaben

• Urheber: Mangiarotti, Agustín ; Aleksanyan, Mina ; Siri, Macarena ; Sun, Tsu‐Wang ; Lipowsky, Reinhard ; Dimova, Rumiana

Weitere Informationen

• DOI: 10.1002/advs.202309864
• URN: urn:nbn:de:101:1-2024040714083961423667
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.