Light‐induced pulsed EPR dipolar spectroscopy on a paradigmatic hemeprotein
Abstract: Light-induced pulsed EPR dipolar spectroscopic methods allow the determination of nanometer distances between paramagnetic sites. Here we employ orthogonal spin labels, a chromophore triplet state and a stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. We demonstrate that Zn-substitution of neuroglobin, to populate the Zn(II) protoporphyrin IX triplet state, makes it possible to perform light-induced pulsed dipolar experiments on hemeproteins, extending the use of light-induced dipolar spectroscopy to this large class of metalloproteins. The versatility of the method is ensured by the employment of different techniques: relaxation-induced dipolar modulation enhancement (RIDME) is applied for the first time to the photoexcited triplet state. In addition, an alternative pulse scheme for laser-induced magnetic dipole (LaserIMD) spectroscopy, based on the refocused-echo detection sequence, is proposed for accurate zero-time determination and reliable distance analysis
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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ChemPhysChem. - 20, 7 (2019) , 931-935, ISSN: 1439-7641
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2020
- Urheber
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Dal Farra, Maria Giulia
Richert, Sabine
Martin, Caterina
Larminie, Charles
Gobbo, Marina
Bergantino, Elisabetta
Timmel, Christiane R.
Bowen, Alice M.
Di Valentin, Marilena
- Beteiligte Personen und Organisationen
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Magnetische Resonanz
- DOI
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10.1002/cphc.201900139
- URN
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urn:nbn:de:bsz:25-freidok-1526534
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:46 MEZ
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Beteiligte
- Dal Farra, Maria Giulia
- Richert, Sabine
- Martin, Caterina
- Larminie, Charles
- Gobbo, Marina
- Bergantino, Elisabetta
- Timmel, Christiane R.
- Bowen, Alice M.
- Di Valentin, Marilena
- Magnetische Resonanz
- Universität
Entstanden
- 2020
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