Light‐induced pulsed EPR dipolar spectroscopy on a paradigmatic hemeprotein
Abstract: Light-induced pulsed EPR dipolar spectroscopic methods allow the determination of nanometer distances between paramagnetic sites. Here we employ orthogonal spin labels, a chromophore triplet state and a stable radical, to carry out distance measurements in singly nitroxide-labeled human neuroglobin. We demonstrate that Zn-substitution of neuroglobin, to populate the Zn(II) protoporphyrin IX triplet state, makes it possible to perform light-induced pulsed dipolar experiments on hemeproteins, extending the use of light-induced dipolar spectroscopy to this large class of metalloproteins. The versatility of the method is ensured by the employment of different techniques: relaxation-induced dipolar modulation enhancement (RIDME) is applied for the first time to the photoexcited triplet state. In addition, an alternative pulse scheme for laser-induced magnetic dipole (LaserIMD) spectroscopy, based on the refocused-echo detection sequence, is proposed for accurate zero-time determination and reliable distance analysis
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
ChemPhysChem. - 20, 7 (2019) , 931-935, ISSN: 1439-7641
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2020
- Urheber
-
Dal Farra, Maria Giulia
Richert, Sabine
Martin, Caterina
Larminie, Charles
Gobbo, Marina
Bergantino, Elisabetta
Timmel, Christiane R.
Bowen, Alice M.
Di Valentin, Marilena
- Beteiligte Personen und Organisationen
-
Magnetische Resonanz
- DOI
-
10.1002/cphc.201900139
- URN
-
urn:nbn:de:bsz:25-freidok-1526534
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:32 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Dal Farra, Maria Giulia
- Richert, Sabine
- Martin, Caterina
- Larminie, Charles
- Gobbo, Marina
- Bergantino, Elisabetta
- Timmel, Christiane R.
- Bowen, Alice M.
- Di Valentin, Marilena
- Magnetische Resonanz
- Universität
Entstanden
- 2020
Ähnliche Objekte (12)
Light‐Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein
Design of an Electrochemical Cell for Continuous Wave EPR Measurements of Radical Ions
Design of an electrochemical cell for continuous wave epr measurements of radical ions
Delocalisation of photoexcited triplet states probed by transient EPR and hyperfine spectroscopy
EPR of photoexcited triplet-state acceptor porphyrins
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Elucidation of the exchange interaction in photoexcited three-spin systems – a second-order perturbational approach
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Calculation of exchange couplings in the electronically excited state of molecular three-spin systems
The effect of the zero-field splitting interaction in light-induced pulsed dipolar EPR spectroscopy
Light‐Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein
Design of an Electrochemical Cell for Continuous Wave EPR Measurements of Radical Ions
Design of an electrochemical cell for continuous wave epr measurements of radical ions
Delocalisation of photoexcited triplet states probed by transient EPR and hyperfine spectroscopy
EPR of photoexcited triplet-state acceptor porphyrins
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Elucidation of the exchange interaction in photoexcited three-spin systems – a second-order perturbational approach
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Calculation of exchange couplings in the electronically excited state of molecular three-spin systems
The effect of the zero-field splitting interaction in light-induced pulsed dipolar EPR spectroscopy
Light‐Induced Pulsed EPR Dipolar Spectroscopy on a Paradigmatic Hemeprotein
Design of an Electrochemical Cell for Continuous Wave EPR Measurements of Radical Ions
Design of an electrochemical cell for continuous wave epr measurements of radical ions
Delocalisation of photoexcited triplet states probed by transient EPR and hyperfine spectroscopy
EPR of photoexcited triplet-state acceptor porphyrins
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Elucidation of the exchange interaction in photoexcited three-spin systems – a second-order perturbational approach
Synthesis and dark state EPR properties of PDI‐trityl dyads and triads
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Chasing the Mond Cation: Synthesis and Characterization of the Homoleptic Nickel Tetracarbonyl Cation and its Tricarbonyl‐Nitrosyl Analogue
Calculation of exchange couplings in the electronically excited state of molecular three-spin systems