Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems

Abstract: Acoustic fields are capable of manipulating biological samples contained within the enclosed and highly controlled environment of a microfluidic chip in a versatile manner. The use of acoustic streaming to alter fluid flows and radiation forces to control cell locations has important clinical and life science applications. While there have been significant advances in the fundamental implementation of these acoustic mechanisms, there is a considerable lack of understanding of the associated biological effects on cells. Typically a single, simple viability assay is used to demonstrate a high proportion of living cells. However, the findings of this study demonstrate that acoustic exposure can inhibit cell attachment, decrease cell spreading, and most intriguingly increase cellular metabolic activity, all without any impact upon viability rates. This has important implications by showing that mortality studies alone are inadequate for the assessment of biocompatibility, but further demonstrates that physical manipulation of cells can also be used to influence their biological activity.

Objekt anzeigen

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Cell Adhesion, Morphology, and Metabolism Variation via Acoustic Exposure within Microfluidic Cell Handling Systems ; volume:6 ; number:24 ; year:2019 ; extent:12 ; Advanced science ; 6, Heft 24 (2019) (gesamt 12)

Beteiligte, Orts- und Zeitangaben

• Urheber: Devendran, Citsabehsan ; Carthew, James ; Frith, Jessica E. ; Neild, Adrian

Weitere Informationen

• DOI: 10.1002/advs.201902326
• URN: urn:nbn:de:101:1-2022071712431746303989
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.