Integration of a bead-based immunoassay on a commercial PCR-performing POC device
Abstract: Point-of-care (POC) infectious disease diagnostic devices typically detect either only pathogens or only protein biomarkers, while they provide a low throughput of samples (one to two) tested per run. In this work, we demonstrate the adaptation of a bead-based competitive immunoassay method to be compatible with a commercial POC PCR-performing device without modifications to its hardware. In addition, the assay integration in two half disk-shaped cartridges with seven identical structures on each provides a considerable throughput of 14 samples per 20 min run
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Notes
-
Proceedings. MDPI. - 97, 1 (2024) , 166, ISSN: 2504-3900
- Event
-
Veröffentlichung
- (where)
-
Freiburg
- (who)
-
Universität
- (when)
-
2024
- Creator
-
Johannsen, Benita
Baumgartner, Desirée
Karpisek, Michal
Stejskal, David
Paust, Nils
Zengerle, Roland
Mitsakakis, Konstantinos
- DOI
-
10.3390/proceedings2024097166
- URN
-
urn:nbn:de:bsz:25-freidok-2493279
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
- 14.08.2025, 11:03 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Johannsen, Benita
- Baumgartner, Desirée
- Karpisek, Michal
- Stejskal, David
- Paust, Nils
- Zengerle, Roland
- Mitsakakis, Konstantinos
- Universität
Time of origin
- 2024
Other Objects (12)
Mikro-Membranpumpen als Komponenten für Mikro-Fluidsysteme
One-step, wash-free, bead-based immunoassay employing bound-free phase detection
ImmunoDisk - a fully automated bead-based immunoassay cartridge with all reagents pre-stored
Water management in the porous transport layers of PEM fuel cells : = Wassermanagement in den porösen Transportschichten von PEM-Brennstoffzellen
Pre-storage of reagents for nucleic acid analysis in unit-use quantities for integration in Lab-on-a-chip test carriers
PMP-NC² - a normally-closed, backpressure independentperistaltic micropump featuring a modular setup : = PMP-NC² - eine normal-geschlossene, Gegendruck unabhängige Mikropumpe mit einem modularen Aufbau
Microfluidics based on silicon, polymer and all-polymer technologies as an alternative to silicon, glass: a case study for TopSpot printheads
Determination of enzyme kinetics in micro concentration gradients : = Bestimmung von Enzym-Kinetiken in Mikro-Konzentrationsgradienten
Advanced centrifugal microfluidics: timing, aliquoting and volume reduction
Highly porous platinum electrodes for the use in potentially implantable glucose fuel cells : = Hochporöse Platinelektroden für den Einsatz in potenziell implantierbaren Glukosebrennstoffzellen
Einweg-Mikro-Membranpumpen mit trennbarer Aktorik zur Insulindosierung
Visualization and modelling of porous media in electrochemical cells : = Visualisierung und Modellierung von porösen Medien in elektrochemischen Zellen
Mikro-Membranpumpen als Komponenten für Mikro-Fluidsysteme
One-step, wash-free, bead-based immunoassay employing bound-free phase detection
ImmunoDisk - a fully automated bead-based immunoassay cartridge with all reagents pre-stored
Water management in the porous transport layers of PEM fuel cells : = Wassermanagement in den porösen Transportschichten von PEM-Brennstoffzellen
Pre-storage of reagents for nucleic acid analysis in unit-use quantities for integration in Lab-on-a-chip test carriers
PMP-NC² - a normally-closed, backpressure independentperistaltic micropump featuring a modular setup : = PMP-NC² - eine normal-geschlossene, Gegendruck unabhängige Mikropumpe mit einem modularen Aufbau
Microfluidics based on silicon, polymer and all-polymer technologies as an alternative to silicon, glass: a case study for TopSpot printheads
Determination of enzyme kinetics in micro concentration gradients : = Bestimmung von Enzym-Kinetiken in Mikro-Konzentrationsgradienten
Advanced centrifugal microfluidics: timing, aliquoting and volume reduction
Highly porous platinum electrodes for the use in potentially implantable glucose fuel cells : = Hochporöse Platinelektroden für den Einsatz in potenziell implantierbaren Glukosebrennstoffzellen
Einweg-Mikro-Membranpumpen mit trennbarer Aktorik zur Insulindosierung
Visualization and modelling of porous media in electrochemical cells : = Visualisierung und Modellierung von porösen Medien in elektrochemischen Zellen
Mikro-Membranpumpen als Komponenten für Mikro-Fluidsysteme
One-step, wash-free, bead-based immunoassay employing bound-free phase detection
ImmunoDisk - a fully automated bead-based immunoassay cartridge with all reagents pre-stored
Water management in the porous transport layers of PEM fuel cells : = Wassermanagement in den porösen Transportschichten von PEM-Brennstoffzellen
Pre-storage of reagents for nucleic acid analysis in unit-use quantities for integration in Lab-on-a-chip test carriers
PMP-NC² - a normally-closed, backpressure independentperistaltic micropump featuring a modular setup : = PMP-NC² - eine normal-geschlossene, Gegendruck unabhängige Mikropumpe mit einem modularen Aufbau
Microfluidics based on silicon, polymer and all-polymer technologies as an alternative to silicon, glass: a case study for TopSpot printheads
Determination of enzyme kinetics in micro concentration gradients : = Bestimmung von Enzym-Kinetiken in Mikro-Konzentrationsgradienten
Advanced centrifugal microfluidics: timing, aliquoting and volume reduction
Highly porous platinum electrodes for the use in potentially implantable glucose fuel cells : = Hochporöse Platinelektroden für den Einsatz in potenziell implantierbaren Glukosebrennstoffzellen
Einweg-Mikro-Membranpumpen mit trennbarer Aktorik zur Insulindosierung