Identification of various cell culture models for the study of Zika virus

Abstract: Aim:
To identify cell culture models supportive for Zika virus
(ZIKV) replication.

Methods:
Various human and non-human cell lines were infected
with a defined amount of ZIKV Polynesia strain. Cells
were analyzed 48 h post infection for the amount
of intracellular and extracellular viral genomes and
infectious viral particles by quantitative real-time PCR
and virus titration assay. The extent of replication was
monitored by immunofluorescence and western blot
analysis by using Env and NS1 specific antibodies.
Innate immunity was assayed by luciferase reporter
assay and immunofluorescence analysis.

Results:
All investigated cell lines except CHO cells supported
infection, replication and release of ZIKV. While in
infected A549 and Vero cells a pronounced cytopathic
effect was observed COS7, 293T and Huh7.5 cells
were most resistant. Although the analyzed cell lines
released comparable amounts of viral genomes to
the supernatant significant differences were found for
the number of infectious viral particles. The neuronal
cell lines N29.1 and SH-SY5Y released 100 times less
infectious viral particles than Vero-, A549- or 293T-cells.
However there is no strict correlation between the
amount of produced viral particles and the induction of
an interferon response in the analyzed cell lines.

Conclusion:
The investigated cell lines with their different tissue
origins and diverging ZIKV susceptibility display a
toolbox for ZIKV research

Objekt anzeigen

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch
• Anmerkungen: World journal of virology. - 7, 1 (2018) , 10-20, ISSN: 2220-3249

Beteiligte, Orts- und Zeitangaben

• Ereignis: Veröffentlichung
• (wo): Freiburg
• (wer): Universität
• (wann): 2019
• Urheber: Himmelsbach, Kiyoshi ; Hildt, Eberhard
• Beteiligte Personen und Organisationen: Universitätsklinikum Freiburg. Klinik für Innere Medizin II

Weitere Informationen

• DOI: 10.5501/wjv.v7.i1.10
• URN: urn:nbn:de:bsz:25-freidok-1449729
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.