Quantifying post-transcriptional regulation in the development of Drosophila melanogaster
Abstract: Even though proteins are produced from mRNA, the correlation between mRNA levels and protein abundances is moderate in most studies, occasionally attributed to complex post-transcriptional regulation. To address this, we generate a paired transcriptome/proteome time course dataset with 14 time points during Drosophila embryogenesis. Despite a limited mRNA-protein correlation (ρ = 0.54), mathematical models describing protein translation and degradation explain 84% of protein time-courses based on the measured mRNA dynamics without assuming complex post transcriptional regulation, and allow for classification of most proteins into four distinct regulatory scenarios. By performing an in-depth characterization of the putatively post-transcriptionally regulated genes, we postulate that the RNA-binding protein Hrb98DE is involved in post-transcriptional control of sugar metabolism in early embryogenesis and partially validate this hypothesis using Hrb98DE knockdown. In summary, we present a systems biology framework for the identification of post-transcriptional gene regulation from large-scale, time-resolved transcriptome and proteome data
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Nature communications. - 9, 1 (2018) , 4970, ISSN: 2041-1723
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2020
- Urheber
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Becker, Kolja
Bluhm, Alina
Casas-Vila, Núria
Dinges, Nadja
Dejung, Mario
Sayols, Sergi
Kreutz, Clemens
Roignant, Jean-Yves
Butter, Falk
Legewie, Stefan
- Beteiligte Personen und Organisationen
- DOI
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10.1038/s41467-018-07455-9
- URN
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urn:nbn:de:bsz:25-freidok-1532110
- Rechteinformation
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Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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25.03.2025, 13:42 MEZ
Datenpartner
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Beteiligte
- Becker, Kolja
- Bluhm, Alina
- Casas-Vila, Núria
- Dinges, Nadja
- Dejung, Mario
- Sayols, Sergi
- Kreutz, Clemens
- Roignant, Jean-Yves
- Butter, Falk
- Legewie, Stefan
- Institut für Medizinische Biometrie und Statistik. Freiburg im Breisgau
- Universität
Entstanden
- 2020
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