Cryo-EM structure of the plant 26S proteasome
Abstract: Targeted proteolysis is a hallmark of life. It is especially important in long-lived cells that can be found in higher eukaryotes, like plants. This task is mainly fulfilled by the ubiquitin–proteasome system. Thus, proteolysis by the 26S proteasome is vital to development, immunity, and cell division. Although the yeast and animal proteasomes are well characterized, there is only limited information on the plant proteasome. We determined the first plant 26S proteasome structure from Spinacia oleracea by single-particle electron cryogenic microscopy at an overall resolution of 3.3 Å. We found an almost identical overall architecture of the spinach proteasome compared with the known structures from mammals and yeast. Nevertheless, we noticed a structural difference in the proteolytic active β1 subunit. Furthermore, we uncovered an unseen compression state by characterizing the proteasome’s conformational landscape. We suspect that this new conformation of the 20S core protease, in correlation with a partial opening of the unoccupied gate, may contribute to peptide release after proteolysis. Our data provide a structural basis for the plant proteasome, which is crucial for further studies
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Plant communications. - 3, 3 (2022) , 100310, ISSN: 2590-3462
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2024
- Creator
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Kandolf, Susanne
Grishkovskaya, Irina
Belacic, Katarina
Bolhuis, Derek L.
Amann, Sascha
Foster, Brent
Imre, Richard
Mechtler, Karl
Schleiffer, Alexander
Tagare, Hemant D.
Zhong, Ellen D.
Meinhart, Anton
Brown, Nicholas G.
Haselbach, David
- DOI
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10.1016/j.xplc.2022.100310
- URN
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urn:nbn:de:bsz:25-freidok-2448713
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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25.03.2025, 1:48 PM CET
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Kandolf, Susanne
- Grishkovskaya, Irina
- Belacic, Katarina
- Bolhuis, Derek L.
- Amann, Sascha
- Foster, Brent
- Imre, Richard
- Mechtler, Karl
- Schleiffer, Alexander
- Tagare, Hemant D.
- Zhong, Ellen D.
- Meinhart, Anton
- Brown, Nicholas G.
- Haselbach, David
- Universität
Time of origin
- 2024
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