A thermosensitive, chitosan-based hydrogel as delivery system for antibacterial liposomes to surgical site infections

Abstract: Prophylaxis and the treatment of surgical site infections (SSIs) with antibiotics frequently fail due to the antibiotic resistance of bacteria and the ability of bacteria to reside in biofilms (i.e., bacterial clusters in a protective matrix). Therefore, alternative antibacterial treatments are required to combat biofilm infections. The combination of diethyldithiocarbamate (DDC−) and copper ions (Cu2+) exhibited antibiofilm activity against the staphylococci species associated with SSIs; however, the formation of a water-insoluble Cu(DDC)2 complex limits its application to SSIs. Here, we describe the development and antibiofilm activity of an injectable gel containing a liposomal formulation of Cu(DDC)2 and Cu2+ (lipogel). Lyophilized liposomes were incorporated into a mixture of chitosan (CS) and beta-glycerophosphate (βGP), and the thermosensitive gelling properties of CS-βGP and the lipogel were determined. The liposomes remained stable after lyophilization over six months at 4–6 °C and −20 °C. The sol-gel transition of the gel and lipogel occurred between 33 and 39 °C, independently of sterilization or storage at −20 °C. CS-βGP is biocompatible and the liposomes were released over time. The lipogel prevented biofilm formation over 2 days and killed 98.7% of the methicillin-resistant Staphylococcus aureus and 99.9% of the Staphylococcus epidermidis biofilms. Therefore, the lipogel is a promising new prophylaxis and treatment strategy for local application to SSIs

Standort
Deutsche Nationalbibliothek Frankfurt am Main
Umfang
Online-Ressource
Sprache
Englisch
Anmerkungen
Pharmaceutics. - 14, 12 (2022) , 2841, ISSN: 1999-4923

Ereignis
Veröffentlichung
(wo)
Freiburg
(wer)
Universität
(wann)
2022
Urheber
Kaul, Laurine
Grundmann, Clara
Köll-Weber, Monika
Löffler, Hanna
Weiz, Artur
Zannettino, Andrew
Richter, Katharina
Süss, Regine

DOI
10.3390/pharmaceutics14122841
URN
urn:nbn:de:bsz:25-freidok-2323555
Rechteinformation
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
Letzte Aktualisierung
25.03.2025, 13:50 MEZ

Datenpartner

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Beteiligte

  • Kaul, Laurine
  • Grundmann, Clara
  • Köll-Weber, Monika
  • Löffler, Hanna
  • Weiz, Artur
  • Zannettino, Andrew
  • Richter, Katharina
  • Süss, Regine
  • Universität

Entstanden

  • 2022

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