Intracellular Molecules Induced Extracellular Peptide Self‐Assembly for Efficient and Effective In Situ Cell Purification
Abstract: Synthetic messenger RNA (mRNA) switches are powerful tools for in situ cell purification, especially for cells derived from stem cells. However, the retention effectiveness of the target cells is limited by the leaky expression of toxic protein. The elimination efficiency of non‐target cells is also constrained due to the lack of signal amplification. In this study, we designed a novel approach that uses synthetic mRNA switch to convey intracellular marker molecule information into spatially controlled extracellular toxic assembly formation. The approach bypasses the use of toxic protein to ensure high target cell recovery effectiveness. Meanwhile, the marker molecule information is amplified at multiple levels to ensure high non‐target cell elimination effectiveness. Our approach can be tailored to meet various in situ cell purification needs, promising high‐quality in situ cell purification for a wide range of biomedical applications.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Intracellular Molecules Induced Extracellular Peptide Self‐Assembly for Efficient and Effective In Situ Cell Purification ; day:04 ; month:08 ; year:2023 ; extent:7
Angewandte Chemie ; (04.08.2023) (gesamt 7)
- Creator
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Li, Cheuk Yin
Liang, Zhenghua
Liu, Lejian
Kuang, Yi
- DOI
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10.1002/ange.202306533
- URN
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urn:nbn:de:101:1-2023080515152746136991
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:45 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Li, Cheuk Yin
- Liang, Zhenghua
- Liu, Lejian
- Kuang, Yi
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Self-assembly of conjugated (macro)molecules
Hijacking Self‐Assembly to Establish Intracellular Functional Nanoparticles
Molecules at work : selfassembly, nanomaterials, molecular machinery
Purification of ethanol for highly sensitive self-assembly experiments
Intracellular and extracellular death regulation : identification of molecules for apoptosis and necroptosis regulation in malignant melanoma
Intracellular and extracellular death regulation: identification of molecules for apoptosis and necroptosis regulation in malignant melanoma
Extracellular Movement of Signaling Molecules
Systematic functionalization of molecules for molecular self-assembly
Systematic functionalization of molecules for molecular self-assembly
Reconfigurable self-assembly of photocatalytic magnetic microrobots for water purification
Gels Obtained by Colloidal Self-Assembly of Amphiphilic Molecules
Intracellular Molecules Induced Extracellular Peptide Self‐Assembly for Efficient and Effective In Situ Cell Purification
Self-assembly of conjugated (macro)molecules
Hijacking Self‐Assembly to Establish Intracellular Functional Nanoparticles
Molecules at work : selfassembly, nanomaterials, molecular machinery
Purification of ethanol for highly sensitive self-assembly experiments
Intracellular and extracellular death regulation : identification of molecules for apoptosis and necroptosis regulation in malignant melanoma
Intracellular and extracellular death regulation: identification of molecules for apoptosis and necroptosis regulation in malignant melanoma
Extracellular Movement of Signaling Molecules
Systematic functionalization of molecules for molecular self-assembly
Systematic functionalization of molecules for molecular self-assembly
Reconfigurable self-assembly of photocatalytic magnetic microrobots for water purification