Yeast Surface Display Enables One‐Step Production and Immobilization of Unspecific Peroxygenases

Abstract: Unspecific peroxygenases (UPOs) are regarded as a “dream catalyst” for selective oxyfunctionalization reactions like oxygenations. We present the display of the model UPO rAaeUPO (PaDa−I) on the cell surface of the heterologous production host Komagataella phaffii as a one‐step production and immobilization process. The coding sequence for PaDa−I was combined with genes coding for cell wall proteins from Saccharomyces cerevisiae and transformed into K. phaffii. The fusion proteins were compared among each other and with secreted, free PaDa−I. One system in particular, a C‐terminal fusion of PaDa−I and Sag1 yielded near identical activity per volume culture broth to the secreted PaDa−I with ~90 % of the activity being at the cell wall. The surface display simplifies downstream processing and includes immobilization on a cheap, retainable and replaceable matrix, that is the production host itself. The enzymes remained active in a repeated batch process for 10 batches and 200 h of catalysis.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Yeast Surface Display Enables One‐Step Production and Immobilization of Unspecific Peroxygenases ; day:22 ; month:08 ; year:2024 ; extent:11 ; ChemCatChem ; (22.08.2024) (gesamt 11)

Beteiligte, Orts- und Zeitangaben

• Urheber: Teetz, Niklas ; Lang, Selina ; Liese, Andreas ; Holtmann, Dirk

Weitere Informationen

• DOI: 10.1002/cctc.202400908
• URN: urn:nbn:de:101:1-2408221433237.814836498720
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 14.08.2025, 10:54 MESZ