INA complex liaises the F1Fo-ATP synthase membrane motor modules
Abstract: The F1F0-ATP synthase translates a proton flux across the inner mitochondrial membrane into a mechanical rotation, driving anhydride bond formation in the catalytic portion. The complex’s membrane-embedded motor forms a proteinaceous channel at the interface between Atp9 ring and Atp6. To prevent unrestricted proton flow dissipating the H+-gradient, channel formation is a critical and tightly controlled step during ATP synthase assembly. Here we show that the INA complex (INAC) acts at this decisive step promoting Atp9-ring association with Atp6. INAC binds to newly synthesized mitochondrial-encoded Atp6 and Atp8 in complex with maturation factors. INAC association is retained until the F1-portion is built on Atp6/8 and loss of INAC causes accumulation of the free F1. An independent complex is formed between INAC and the Atp9 ring. We conclude that INAC maintains assembly intermediates of the F1 F0-ATP synthase in a primed state for the terminal assembly step–motor module formation
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
Nature communications. - 8, 1 (2017) , 1237, ISSN: 2041-1723
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2019
- Urheber
- DOI
-
10.1038/s41467-017-01437-z
- URN
-
urn:nbn:de:bsz:25-freidok-1411114
- Rechteinformation
-
Kein Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
14.08.2025, 11:04 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Naumenko, Nataliia
- Morgenstern, Marcel
- Rucktäschel, Robert
- Warscheid, Bettina
- Rehling, Peter
- Universität
Entstanden
- 2019
Ähnliche Objekte (12)
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Protein transport along the presequence pathway
Function of the INA complex in assembly of the mitochondrial oxidative phosphorylation system
From TOM to the TIM23 complex – handing over of a precursor
Defining the substrate spectrum of the TIM22 complex identifies pyruvate carrier subunits as unconventional cargos
Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei
MITRAC7 acts as a COX1-specific chaperone and reveals a checkpoint during cytochrome c oxidase assembly
Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
Analysis of Mdm38 function in mitochondrial translation
Mitochondrial protein assemblies: Biogenesis of the cytochrome c oxidase and mitophagic signaling complexes
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Protein transport along the presequence pathway
Function of the INA complex in assembly of the mitochondrial oxidative phosphorylation system
From TOM to the TIM23 complex – handing over of a precursor
Defining the substrate spectrum of the TIM22 complex identifies pyruvate carrier subunits as unconventional cargos
Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei
MITRAC7 acts as a COX1-specific chaperone and reveals a checkpoint during cytochrome c oxidase assembly
Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
Analysis of Mdm38 function in mitochondrial translation
Mitochondrial protein assemblies: Biogenesis of the cytochrome c oxidase and mitophagic signaling complexes
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Massenspektrometrische Untersuchungen zur chemischen Charakterisierung sekundärer organischer Aerosole und ihrer Bildungsprozesse in der Troposphäre
Protein transport along the presequence pathway
Function of the INA complex in assembly of the mitochondrial oxidative phosphorylation system
From TOM to the TIM23 complex – handing over of a precursor
Defining the substrate spectrum of the TIM22 complex identifies pyruvate carrier subunits as unconventional cargos
Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei
MITRAC7 acts as a COX1-specific chaperone and reveals a checkpoint during cytochrome c oxidase assembly
Study of Z-disc-associated signaling networks in skeletal muscle cells by functional and global phosphoproteomics
CRISPR-Cas9-mediated protein tagging in human cells for RESOLFT nanoscopy and the analysis of mitochondrial prohibitins
Analysis of Mdm38 function in mitochondrial translation