Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations

Abstract: The bacterial Min protein system was encapsulated in giant unilamellar vesicles (GUVs). Using confocal fluorescence microscopy, we identified several distinct modes of spatiotemporal patterns inside spherical GUVs. For osmotically deflated GUVs, the vesicle shape actively changed in concert with the Min oscillations. The periodic relocation of Min proteins from the vesicle lumen to the membrane and back is accompanied by drastic changes in the mechanical properties of the lipid bilayer. In particular, two types of oscillating membrane‐shape changes are highlighted: 1) GUVs that repeatedly undergo fission into two connected compartments and fusion of these compartments back into a dumbbell shape and 2) GUVs that show periodic budding and subsequent merging of the buds with the mother vesicle, accompanied by an overall shape change of the vesicle reminiscent of a bouncing ball. These findings demonstrate how reaction–diffusion‐based protein self‐organization can directly yield visible mechanical effects on membrane compartments, even up to autonomous division, without the need for coupling to cytoskeletal elements.

Angaben zum Objekt

• Standort: Deutsche Nationalbibliothek Frankfurt am Main
• Umfang: Online-Ressource
• Sprache: Englisch

Verweise und Beziehungen

• Erschienen in: Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations ; volume:57 ; number:50 ; year:2018 ; pages:16286-16290 ; extent:5 ; Angewandte Chemie / International edition. International edition ; 57, Heft 50 (2018), 16286-16290 (gesamt 5)

Beteiligte, Orts- und Zeitangaben

• Urheber: Litschel, Thomas ; Ramm, Beatrice ; Maas, Roel ; Heymann, Michael ; Schwille, Petra

Weitere Informationen

• DOI: 10.1002/anie.201808750
• URN: urn:nbn:de:101:1-2022090620391088537545
• Rechteinformation: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Letzte Aktualisierung: 15.08.2025, 07:33 MESZ