Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes
Abstract: One approach to bringing enzymes together for multienzyme biocatalysis is genetic fusion. This enables the production of multifunctional enzymes that can be used for whole‐cell biotransformations or for in vitro (cascade) reactions. In some cases and in some aspects, such as expression and conversions, the fused enzymes outperform a combination of the individual enzymes. In contrast, some enzyme fusions are greatly compromised in activity and/or expression. In this Minireview, we give an overview of studies on fusions between two or more enzymes that were used for biocatalytic applications, with a focus on oxidative enzymes. Typically, the enzymes are paired to facilitate cofactor recycling or cosubstrate supply. In addition, different linker designs are briefly discussed. Although enzyme fusion is a promising tool for some biocatalytic applications, future studies could benefit from integrating the findings of previous studies in order to improve reliability and effectiveness.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Enzyme Fusions in Biocatalysis: Coupling Reactions by Pairing Enzymes ; volume:20 ; number:1 ; year:2019 ; pages:20-28 ; extent:9
ChemBioChem ; 20, Heft 1 (2019), 20-28 (gesamt 9)
- Urheber
- DOI
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10.1002/cbic.201800394
- URN
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urn:nbn:de:101:1-2022071407045084133570
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:27 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Aalbers, Friso S.
- Fraaije, Marco Wilhelmus
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