Engineered Lipids for Intracellular Reactive Oxygen Species Scavenging in Steatotic Hepatocytes

Abstract: Intracellular reactive oxygen species (ROS) in steatotic cells pose a problem due to their potential to cause oxidative stress and cellular damage. Delivering engineered phospholipids to intracellular lipid droplets in steatotic hepatic cells, using the cell's inherent intracellular lipid transport mechanisms are investigated. Initially, it is shown that tail‐labeled fluorescent lipids assembled into liposomes are able to be transported to intracellular lipid droplets in steatotic HepG2 cells and HHL‐5 cells. Further, an antioxidant, an EUK salen–manganese derivative, which has superoxide dismutase‐like and catalase‐like activity, is covalently conjugated to the tail of a phospholipid and formulated as liposomes for administration. Steatotic HepG2 cells and HHL‐5 cells incubated with these antioxidant liposomes have lower intracellular ROS levels compared to untreated controls and non‐covalently formulated antioxidants. This first proof‐of‐concept study illustrates an alternative strategy to equip native organelles in mammalian cells with engineered enzyme activity.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Engineered Lipids for Intracellular Reactive Oxygen Species Scavenging in Steatotic Hepatocytes ; day:01 ; month:07 ; year:2024 ; extent:15 ; Small ; (01.07.2024) (gesamt 15)

Contributors, Places and Time

• Creator: Westensee, Isabella N. ; de Dios Andres, Paula ; Brodszkij, Edit ; Descours, Pierre‐Louis ; Perez‐Rodriguez, Diego ; Spinazzola, Antonella ; Mookerjee, Rajeshwar Prosad ; Städler, Brigitte

Further information

• DOI: 10.1002/smll.202400816
• URN: urn:nbn:de:101:1-2407011433085.602897317691
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 11:01 AM CEST