Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry
Exosomes are regarded as the emerging potential targets for liquid biopsy and bioprocess study owing to their abundant inclusive cargos that carry significant disease information. In addition, metabolites have been promising biomarkers for diagnosis. However, little metabolic research on exosomes is carried out by now. Herein, the mix‐crystal titania‐assisted laser desorption/ionization mass spectrometry (LDI‐MS) method is established, which features fast speed, high throughput, and efficiency, to directly extract urinary exosome metabolic patterns of healthy controls (HC) and membrane nephropathy (MN) patients. Besides, this method is also adopted to acquire the primitive urinary metabolic patterns from the same samples for comparison. By taking advantage of principal component analysis, unpaired parametric t‐test, and orthogonal partial least squares discriminant analysis on the exosome metabolic patterns, 27 significant m/z signals are filtrated, which possess more prominent differentiation capacity toward HC and MN patients (AUC = 0.942), and hold greater potential in MN diagnosis, compared to primitive urine (AUC = 0.801). The work reveals the important clinical value of exosome metabolic analysis, and paves a way to exosome‐based diagnosis at metabolomic level toward large‐scale clinical use.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Investigation of Urinary Exosome Metabolic Patterns in Membranous Nephropathy by Titania‐Assisted Intact Exosome Mass Spectrometry ; day:09 ; month:02 ; year:2022 ; extent:8
Small science ; (09.02.2022) (gesamt 8)
- Creator
-
Chen, Haolin
Zhang, Ning
Wu, Yonglei
Yang, Chenjie
Xie, Qionghong
Deng, Chunhui
Sun, Nianrong
- DOI
-
10.1002/smsc.202100118
- URN
-
urn:nbn:de:101:1-2022021014000808592827
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:27 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Chen, Haolin
- Zhang, Ning
- Wu, Yonglei
- Yang, Chenjie
- Xie, Qionghong
- Deng, Chunhui
- Sun, Nianrong
Other Objects (12)
Promoting the bench-to-bedside translation of nanomedicines
Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration
Special issue of Sustainable Asia Conference 2014
Infinitely many geometrically distinct solutions for periodic Schrödinger–Poisson systems
An integrated approach for knowledge extraction and analysis in collaborative knowledge construction
Blending SMAP, Noah and In Situ Soil Moisture Using Multiple Methods
Mountain-pass-type solutions for Schrödinger equations in R2 with unbounded or vanishing potentials and critical exponential growth nonlinearities
Coordinate Ellipsis and Information Structure
Nanoscale self-healing mechanisms in shape memory ceramics
Exosome-orchestrated hypoxic tumor microenvironment
CRABP1-complexes in exosome secretion
Exosome markers of LRRK2 kinase inhibition
Promoting the bench-to-bedside translation of nanomedicines
Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration
Special issue of Sustainable Asia Conference 2014
Infinitely many geometrically distinct solutions for periodic Schrödinger–Poisson systems
An integrated approach for knowledge extraction and analysis in collaborative knowledge construction
Blending SMAP, Noah and In Situ Soil Moisture Using Multiple Methods
Mountain-pass-type solutions for Schrödinger equations in R2 with unbounded or vanishing potentials and critical exponential growth nonlinearities
Coordinate Ellipsis and Information Structure
Nanoscale self-healing mechanisms in shape memory ceramics
Exosome-orchestrated hypoxic tumor microenvironment
CRABP1-complexes in exosome secretion
Exosome markers of LRRK2 kinase inhibition
Promoting the bench-to-bedside translation of nanomedicines
Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration
Special issue of Sustainable Asia Conference 2014
Infinitely many geometrically distinct solutions for periodic Schrödinger–Poisson systems
An integrated approach for knowledge extraction and analysis in collaborative knowledge construction
Blending SMAP, Noah and In Situ Soil Moisture Using Multiple Methods
Mountain-pass-type solutions for Schrödinger equations in R2 with unbounded or vanishing potentials and critical exponential growth nonlinearities
Coordinate Ellipsis and Information Structure
Nanoscale self-healing mechanisms in shape memory ceramics
Exosome-orchestrated hypoxic tumor microenvironment
CRABP1-complexes in exosome secretion