Proteoform‐Selective Imaging of Tissues Using Mass Spectrometry **
Abstract: Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform‐specific analysis of the proteome. Herein, we employ nanospray desorption electrospray ionization mass spectrometry imaging (nano‐DESI MSI) for the proteoform‐selective imaging of biological tissues. Nano‐DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS) directly on the tissue. Proof‐of‐concept experiments demonstrate that nano‐DESI MSI combined with on‐tissue top‐down proteomics is ideally suited for the proteoform‐selective imaging of tissue sections. Using rat brain tissue as a model system, we provide the first evidence of differential proteoform expression in different regions of the brain.
- Location
-
Deutsche Nationalbibliothek Frankfurt am Main
- Extent
-
Online-Ressource
- Language
-
Englisch
- Bibliographic citation
-
Proteoform‐Selective Imaging of Tissues Using Mass Spectrometry ** ; day:18 ; month:05 ; year:2022 ; extent:1
Angewandte Chemie ; (18.05.2022) (gesamt 1)
- Creator
-
Yang, Manxi
Hu, Hang
Su, Pei
Thomas, Paul M.
Camarillo, Jeannie M.
Greer, Joseph B.
Early, Bryan P.
Fellers, Ryan T.
Kelleher, Neil L.
Laskin, Julia
- DOI
-
10.1002/ange.202200721
- URN
-
urn:nbn:de:101:1-2022051915025084478474
- Rights
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
-
15.08.2025, 7:32 AM CEST
Data provider
This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Yang, Manxi
- Hu, Hang
- Su, Pei
- Thomas, Paul M.
- Camarillo, Jeannie M.
- Greer, Joseph B.
- Early, Bryan P.
- Fellers, Ryan T.
- Kelleher, Neil L.
- Laskin, Julia
Other Objects (12)
Proteoform‐Selective Imaging of Tissues Using Mass Spectrometry **
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
Round robin study of formalin-fixed paraffin-embedded tissues in mass spectrometry imaging
Are Phosphatidic Acids Ubiquitous in Mammalian Tissues or Overemphasized in Mass Spectrometry Imaging Applications?
In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues
Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging
Chemical imaging of biological tissues
Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method
Imaging the uptake, distribution and metabolism of agrochemicals using MALDI mass spectrometry imaging
Probing molecular tracers in geobiological systems using imaging mass spectrometry
Proteoform‐Selective Imaging of Tissues Using Mass Spectrometry **
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
Round robin study of formalin-fixed paraffin-embedded tissues in mass spectrometry imaging
Are Phosphatidic Acids Ubiquitous in Mammalian Tissues or Overemphasized in Mass Spectrometry Imaging Applications?
In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues
Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging
Chemical imaging of biological tissues
Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method
Imaging the uptake, distribution and metabolism of agrochemicals using MALDI mass spectrometry imaging
Probing molecular tracers in geobiological systems using imaging mass spectrometry
Proteoform‐Selective Imaging of Tissues Using Mass Spectrometry **
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
MALDI imaging mass spectrometry in clinical proteomics research of gastric cancer tissues
Mass Spectrometry Imaging and Integration with Other Imaging Modalities for Greater Molecular Understanding of Biological Tissues
Round robin study of formalin-fixed paraffin-embedded tissues in mass spectrometry imaging
Are Phosphatidic Acids Ubiquitous in Mammalian Tissues or Overemphasized in Mass Spectrometry Imaging Applications?
In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues
Tissue derivatization for visualizing lactate and pyruvate in mouse testis tissues using matrix-assisted laser desorption/ionization-mass spectrometry imaging
Chemical imaging of biological tissues
Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method
Imaging the uptake, distribution and metabolism of agrochemicals using MALDI mass spectrometry imaging