A Double‐Armed, Hydrophilic Transition Metal Complex as a Paramagnetic NMR Probe
Abstract: Synthetic metal complexes can be used as paramagnetic probes for the study of proteins and protein complexes. Herein, two transition metal NMR probes (TraNPs) are reported. TraNPs are attached through two arms to a protein to generate a pseudocontact shift (PCS) using cobalt (II), or paramagnetic relaxation enhancement (PRE) with manganese (II). The PCS analysis of TraNPs attached to three different proteins shows that the size of the anisotropic component of the magnetic susceptibility depends on the probe surroundings at the surface of the protein, contrary to what is observed for lanthanoid‐based probes. The observed PCS are relatively small, making cobalt‐based probes suitable for localized studies, such as of an active site. The obtained PREs are stronger than those obtained with nitroxide spin labels and the possibility to generate both PCS and PRE offers advantages. The properties of TraNPs in comparison with other cobalt‐based probes are discussed.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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A Double‐Armed, Hydrophilic Transition Metal Complex as a Paramagnetic NMR Probe ; volume:131 ; number:37 ; year:2019 ; pages:13227-13234 ; extent:8
Angewandte Chemie ; 131, Heft 37 (2019), 13227-13234 (gesamt 8)
- Urheber
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Miao, Qing
Liu, Wei‐Min
Kock, Thomas
Blok, Anneloes
Timmer, Monika
Overhand, Mark
Ubbink, Marcellus
- DOI
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10.1002/ange.201906049
- URN
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urn:nbn:de:101:1-2022080106482015694893
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:34 MESZ
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Beteiligte
- Miao, Qing
- Liu, Wei‐Min
- Kock, Thomas
- Blok, Anneloes
- Timmer, Monika
- Overhand, Mark
- Ubbink, Marcellus
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