Biomimetic S ‐Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes **

to related objects

Abstract: S‐Adenosylmethionine (SAM) is an enzyme cofactor involved in methylation, aminopropyl transfer, and radical reactions. This versatility renders SAM‐dependent enzymes of great interest in biocatalysis. The usage of SAM analogues adds to this diversity. However, high cost and instability of the cofactor impedes the investigation and usage of these enzymes. While SAM regeneration protocols from the methyltransferase (MT) byproduct S‐adenosylhomocysteine are available, aminopropyl transferases and radical SAM enzymes are not covered. Here, we report a set of efficient one‐pot systems to supply or regenerate SAM and SAM analogues for all three enzyme classes. The systems’ flexibility is showcased by the transfer of an ethyl group with a cobalamin‐dependent radical SAM MT using S‐adenosylethionine as a cofactor. This shows the potential of SAM (analogue) supply and regeneration for the application of diverse chemistry, as well as for mechanistic studies using cofactor analogues.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Biomimetic S ‐Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes ** ; day:04 ; month:04 ; year:2023 ; extent:12
ChemBioChem ; (04.04.2023) (gesamt 12)

Creator: Gericke, Lukas
Mhaindarkar, Dipali
Karst, Lukas C.
Jahn, Sören
Kuge, Marco
Mohr, Michael K. F.
Gagsteiger, Jana
Cornelissen, Nicolas V.
Wen, Xiaojin
Mordhorst, Silja
Jessen, Henning Jacob
Rentmeister, Andrea
Seebeck, Florian Peter
Layer, Gunhild
Lönarz, Christoph
Andexer, Jennifer Nina

DOI: 10.1002/cbic.202300133

URN: urn:nbn:de:101:1-2023040415280566038266

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 14.08.2025, 11:01 AM CEST

Data provider

This object is provided by:
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.

Show original at data provider

Associated

Gericke, Lukas
Mhaindarkar, Dipali
Karst, Lukas C.
Jahn, Sören
Kuge, Marco
Mohr, Michael K. F.
Gagsteiger, Jana
Cornelissen, Nicolas V.
Wen, Xiaojin
Mordhorst, Silja
Jessen, Henning Jacob
Rentmeister, Andrea
Seebeck, Florian Peter
Layer, Gunhild
Lönarz, Christoph
Andexer, Jennifer Nina

Other Objects (12)

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Hochschulschrift

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

The 8th young faculty meeting – an active crowd attuned to modern challenges

The 8th young faculty meeting – an active crowd attuned to modern challenges

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Design of sensors for highly phosphorylated natural compounds

Design of sensors for highly phosphorylated natural compounds

Rapid synthesis of nucleoside triphosphates and analogues

Rapid synthesis of nucleoside triphosphates and analogues

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Hochschulschrift

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

The 8th young faculty meeting – an active crowd attuned to modern challenges

The 8th young faculty meeting – an active crowd attuned to modern challenges

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Design of sensors for highly phosphorylated natural compounds

Design of sensors for highly phosphorylated natural compounds

Rapid synthesis of nucleoside triphosphates and analogues

Rapid synthesis of nucleoside triphosphates and analogues

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Hochschulschrift

Untersuchungen zur intrazellulären Freisetzung von Nucleosidphosphaten

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

Biomimetic S-adenosylmethionine regeneration starting from multiple byproducts enables biocatalytic alkylation with radical sam enzymes

The 8th young faculty meeting – an active crowd attuned to modern challenges

The 8th young faculty meeting – an active crowd attuned to modern challenges

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Synthesis of bisdiphosphoinositol polyphosphate prometabolites

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Investigations on the synthesis and degradation of polyphosphate in vitro and its correlation to magic spot nucleotides

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Development of novel p-amidites and their application in the synthesis of the most densely phosphorylated compounds

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Novel shades of inositol pyrophosphates – in vitro and in cellulo evaluation of inositol pyrophosphate probes

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Cyclotriphosphate: A Brief History, Recent Developments, and Perspectives in Synthesis

Design of sensors for highly phosphorylated natural compounds

Design of sensors for highly phosphorylated natural compounds

Rapid synthesis of nucleoside triphosphates and analogues

Rapid synthesis of nucleoside triphosphates and analogues

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Multiple Light Control Mechanisms in ATP‐Fueled Non‐equilibrium DNA Systems

Cultural heritage institutions wishing to register will find more information here.

Fields marked * need to be filled in.

Username*

Please enter your username

Email*

Please enter your email address

Please do not fill this field

First name

Last name

Password*

Please enter your password

Confirm password*

Please enter the same password

I have read the terms of use and the privacy policy for the collection of personal data and accept them. *

This field is required.

I would like to subscribe to the newsletter of the Deutsche Digitale Bibliothek. See newsletter subscription info.

Account created

Your "My DDB" account has been successfully created. Before you can log in to your account, you must click the confirmation link in the message we just sent to the email address you provided.