A Detailed View on Geosmin Biosynthesis

Abstract: The bacterial geosmin synthase is a fascinating bifunctional enzyme that has been discovered almost two decades ago. Several aspects of the cyclisation mechanism from FPP to geosmin are known, but a detailed picture of the stereochemical course of this reaction is unknown. This article reports on a deep investigation of the mechanism of geosmin synthase through isotopic labelling experiments. Furthermore, the effects of divalent cations on geosmin synthase catalysis were investigated. The addition of cyclodextrin to enzymatic reactions, a molecule that can capture terpenes, suggests that the biosynthetic intermediate (1 (10) E,5E)‐germacradien‐11‐ol produced by the N‐terminal domain is passed to the C‐terminal domain not through a tunnel, but rather through release into the medium and uptake by the C‐terminal domain.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: A Detailed View on Geosmin Biosynthesis ; day:16 ; month:05 ; year:2023 ; extent:7 ; ChemBioChem ; (16.05.2023) (gesamt 7)

Contributors, Places and Time

• Creator: Xu, Houchao ; Dickschat, Jeroen S.

Further information

• DOI: 10.1002/cbic.202300101
• URN: urn:nbn:de:101:1-2023051615452484005121
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:46 AM CEST