Ultrastructural Imaging of Salmonella –Host Interactions Using Super‐resolution Correlative Light‐Electron Microscopy of Bioorthogonal Pathogens
Abstract: The imaging of intracellular pathogens inside host cells is complicated by the low resolution and sensitivity of fluorescence microscopy and by the lack of ultrastructural information to visualize the pathogens. Herein, we present a new method to visualize these pathogens during infection that circumvents these problems: by using a metabolic hijacking approach to bioorthogonally label the intracellular pathogen Salmonella Typhimurium and by using these bioorthogonal groups to introduce fluorophores compatible with stochastic optical reconstruction microscopy (STORM) and placing this in a correlative light electron microscopy (CLEM) workflow, the pathogen can be imaged within its host cell context Typhimurium with a resolution of 20 nm. This STORM‐CLEM approach thus presents a new approach to understand these pathogens during infection.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Ultrastructural Imaging of Salmonella –Host Interactions Using Super‐resolution Correlative Light‐Electron Microscopy of Bioorthogonal Pathogens ; volume:19 ; number:16 ; year:2018 ; pages:1766-1770 ; extent:5
ChemBioChem ; 19, Heft 16 (2018), 1766-1770 (gesamt 5)
- Creator
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van Elsland, Daphne M.
Pujals, Sílvia
Bakkum, Thomas
Bos, Erik
Oikonomeas‐Koppasis, Nikolaos
Berlin, Ilana
Neefjes, Jacques
Meijer, Annemarie H.
Koster, Abraham J.
Albertazzi, Lorenzo
van Kasteren, Sander I.
- DOI
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10.1002/cbic.201800230
- URN
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urn:nbn:de:101:1-2022081606565790312326
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:37 AM CEST
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Associated
- van Elsland, Daphne M.
- Pujals, Sílvia
- Bakkum, Thomas
- Bos, Erik
- Oikonomeas‐Koppasis, Nikolaos
- Berlin, Ilana
- Neefjes, Jacques
- Meijer, Annemarie H.
- Koster, Abraham J.
- Albertazzi, Lorenzo
- van Kasteren, Sander I.
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