Protein O-glycosylation analysis
Abstract: This review provides an overview on the methods available for analysis of O-glycosylation. Three major themes are addressed: analysis of released O-glycans including different O-glycan liberation, derivatization, and detection methods; analysis of formerly O-glycosylated peptides yielding information on O-glycan attachment sites; analysis of O-glycopeptides, representing by far the most informative but also most challenging approach for O-glycan analysis. Although there are various techniques available for the identification of O-linked oligosaccharides, the focus here is on MS fragmentation techniques such as collision-induced fragmentation, electron capture dissociation, and electron transfer dissociation. Finally, the O-glycan analytical challenges that need to be met will be discussed.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Protein O-glycosylation analysis ; volume:393 ; number:8 ; year:2012 ; pages:687-708
Biological chemistry ; 393, Heft 8 (2012), 687-708
- Urheber
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Zauner, Gerhild
Kozak, Radoslaw P.
Gardner, Richard A.
Fernandes, Daryl L.
Deelder, André M.
Wuhrer, Manfred
- DOI
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10.1515/hsz-2012-0144
- URN
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urn:nbn:de:101:1-2408051712172.683511746752
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 10:59 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Zauner, Gerhild
- Kozak, Radoslaw P.
- Gardner, Richard A.
- Fernandes, Daryl L.
- Deelder, André M.
- Wuhrer, Manfred
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