A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis
Abstract: A discrete acyl carrier protein (ACP) bearing a photolabile nonhydrolysable carba (dethia) malonyl pantetheine cofactor was chemoenzymatically prepared and utilised for the trapping of biosynthetic polyketide intermediates following light activation. From the in vitro assembly of the polyketides SEK4 and SEK4b, by the type II actinorhodin “minimal” polyketide synthase (PKS), a range of putative ACP‐bound diketides, tetraketides, pentaketides and hexaketides were identified and characterised by FT‐ICR‐MS, providing direct insights on active site accessibility and substrate processing for this enzyme class.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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A Light‐Activated Acyl Carrier Protein “Trap” for Intermediate Capture in Type II Iterative Polyketide Biocatalysis ; volume:25 ; number:72 ; year:2019 ; pages:16515-16518 ; extent:4
Chemistry - a European journal ; 25, Heft 72 (2019), 16515-16518 (gesamt 4)
- Urheber
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Kilgour, Samantha L.
Kilgour, David P. A.
Prasongpholchai, Panward
O'Connor, Peter B.
Tosin, Manuela
- DOI
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10.1002/chem.201903662
- URN
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urn:nbn:de:101:1-2022072408035894664792
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:31 MESZ
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Beteiligte
- Kilgour, Samantha L.
- Kilgour, David P. A.
- Prasongpholchai, Panward
- O'Connor, Peter B.
- Tosin, Manuela
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