Single‐Cell Spatial Transcriptomics Unveils Platelet‐Fueled Cycling Macrophages for Kidney Fibrosis
Abstract: With the increasing incidence of kidney diseases, there is an urgent need to develop therapeutic strategies to combat post‐injury fibrosis. Immune cells, including platelets, play a pivotal role in this repair process, primarily through their released cytokines. However, the specific role of platelets in kidney injury and subsequent repair remains underexplored. Here, the detrimental role of platelets in renal recovery following ischemia/reperfusion injury and its contribution to acute kidney injury to chronic kidney disease transition is aimed to investigated. In this study, it is shown that depleting platelets accelerates injury resolution and significantly reduces fibrosis. Employing advanced single‐cell and spatial transcriptomic techniques, macrophages as the primary mediators modulated by platelet signals is identified. A novel subset of macrophages, termed “cycling M2”, which exhibit an M2 phenotype combined with enhanced proliferative activity is uncovered. This subset emerges in the injured kidney during the resolution phase and is modulated by platelet‐derived thrombospondin 1 (THBS1) signaling, acquiring profibrotic characteristics. Conversely, targeted inhibition of THBS1 markedly downregulates the cycling M2 macrophage, thereby mitigating fibrotic progression. Overall, this findings highlight the adverse role of platelet THBS1‐boosted cycling M2 macrophages in renal injury repair and suggest platelet THBS1 as a promising therapeutic target for alleviating inflammation and kidney fibrosis.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Single‐Cell Spatial Transcriptomics Unveils Platelet‐Fueled Cycling Macrophages for Kidney Fibrosis ; day:05 ; month:06 ; year:2024 ; extent:19
Advanced science ; (05.06.2024) (gesamt 19)
- Creator
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Liu, Jun
Zheng, Bo
Cui, Qingya
Zhu, Yu
Chu, Likai
Geng, Zhi
Mao, Yiming
Wan, Lin
Cao, Xu
Xiong, Qianwei
Guo, Fujia
Yang, David C.
Hsu, Ssu‐Wei
Chen, Ching‐Hsien
Yan, Xiangming
- DOI
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10.1002/advs.202308505
- URN
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urn:nbn:de:101:1-2406061403293.709836863879
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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14.08.2025, 10:46 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Liu, Jun
- Zheng, Bo
- Cui, Qingya
- Zhu, Yu
- Chu, Likai
- Geng, Zhi
- Mao, Yiming
- Wan, Lin
- Cao, Xu
- Xiong, Qianwei
- Guo, Fujia
- Yang, David C.
- Hsu, Ssu‐Wei
- Chen, Ching‐Hsien
- Yan, Xiangming
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