Expression of human growth hormone gene in Pleurotus eryngii
Abstract: Recombinant human growth hormone (rhGH) has been widely used in many medical applications. Large scale production of rhGH is important for a wider application of this molecule in the field of proteomics. This investigation reports a modified Agrobacterium-mediated method for transfer and expression of human growth hormone gene in the popular edible mushroom Pleurotus eryngii. A binary vector pCAMBIA1304 containing the hGH2 gene was constructed and introduced into Pleurotus eryngii via Agrobacterium tumefaciens-mediated transformation. Integration of hGH2 into mushroom genome was detected by PCR and expression was confirmed by Western blot assay. The successful expression of the human growth hormone gene in mushroom suggests that the proposed modified transformation system is probably useful for the production of transgenic mushrooms.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Expression of human growth hormone gene in Pleurotus eryngii ; volume:5 ; number:6 ; year:2010 ; pages:791-799 ; extent:9
Open life sciences ; 5, Heft 6 (2010), 791-799 (gesamt 9)
- Urheber
-
Kim, Seung
Sapkota, Kumar
Choi, Bong-Suk
Kim, Sung-Jun
- DOI
-
10.2478/s11535-010-0071-8
- URN
-
urn:nbn:de:101:1-2409201859211.677745026042
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:35 MESZ
Datenpartner
Dieses Objekt wird bereitgestellt von:
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Kim, Seung
- Sapkota, Kumar
- Choi, Bong-Suk
- Kim, Sung-Jun
Ähnliche Objekte (12)
Growth hormone over the human life span
Structural analysis of human growth hormone with respect to the dominant expression of GH mutations in isolated growth hormone deficiency type II
In vitro characterization of human growth hormone mutants
Exopolysaccharides from Pseudomonas tolaasii inhibit the growth of Pleurotus ostreatus mycelia
Growth response and mycoremediation of heavy metals by fungus Pleurotus sp
Antioxidant capacity of Pleurotus ostreatus (Jacq.) P. Kumm influenced by growth substrates
The use of recombinant human growth hormone in patients with Mucopolysaccharidoses and growth hormone deficiency: a case series
Das ligninolytische System von Pleurotus sapidus: Transkriptomanalyse und heterologe Expression einer Arylalkoholoxidase
Effects of recombinant human growth hormone administration on cardiovascular risk factors in obese children with relative growth hormone deficiency
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
Growth hormone – releasing hormone in the immune system
Growth hormone over the human life span
Structural analysis of human growth hormone with respect to the dominant expression of GH mutations in isolated growth hormone deficiency type II
In vitro characterization of human growth hormone mutants
Exopolysaccharides from Pseudomonas tolaasii inhibit the growth of Pleurotus ostreatus mycelia
Growth response and mycoremediation of heavy metals by fungus Pleurotus sp
Antioxidant capacity of Pleurotus ostreatus (Jacq.) P. Kumm influenced by growth substrates
The use of recombinant human growth hormone in patients with Mucopolysaccharidoses and growth hormone deficiency: a case series
Das ligninolytische System von Pleurotus sapidus: Transkriptomanalyse und heterologe Expression einer Arylalkoholoxidase
Effects of recombinant human growth hormone administration on cardiovascular risk factors in obese children with relative growth hormone deficiency
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
Growth hormone – releasing hormone in the immune system
Growth hormone over the human life span
Structural analysis of human growth hormone with respect to the dominant expression of GH mutations in isolated growth hormone deficiency type II
In vitro characterization of human growth hormone mutants
Exopolysaccharides from Pseudomonas tolaasii inhibit the growth of Pleurotus ostreatus mycelia
Growth response and mycoremediation of heavy metals by fungus Pleurotus sp
Antioxidant capacity of Pleurotus ostreatus (Jacq.) P. Kumm influenced by growth substrates
The use of recombinant human growth hormone in patients with Mucopolysaccharidoses and growth hormone deficiency: a case series
Das ligninolytische System von Pleurotus sapidus: Transkriptomanalyse und heterologe Expression einer Arylalkoholoxidase
Effects of recombinant human growth hormone administration on cardiovascular risk factors in obese children with relative growth hormone deficiency
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis
A minimal growth medium for the basidiomycete Pleurotus sapidus for metabolic flux analysis