Microengineered Multi‐Organoid System from hiPSCs to Recapitulate Human Liver‐Islet Axis in Normal and Type 2 Diabetes
Abstract: Type 2 diabetes mellitus (T2DM) is a systematic multi‐organ metabolic disease, which is characterized by the dynamic interplay among different organs. The increasing incidence of T2DM reflects an urgent need for the development of in vitro human‐relevant models for disease study and drug therapy. Here, a new microfluidic multi‐organoid system is developed that recapitulates the human liver‐pancreatic islet axis in normal and disease states. The system contains two compartmentalized regions connected by a microchannel network, enabling 3D co‐culture of human induced pluripotent stem cells (hiPSC)‐derived liver and islet organoids for up to 30 days under circulatory perfusion conditions. The co‐cultured liver and islet organoids exhibit favorable growth and improved tissue‐specific functions. Transcriptional analyses reveal the activation of metabolically relevant signaling pathways in the co‐cultured organoids. Notably, the co‐culture system facilitates sensitive glucose‐stimulated insulin secretion from islet organoids and increased glucose utilization in liver organoids by glucose tolerance tests. Both liver and islet organoids display mitochondrial dysfunction and decreased glucose transport capacity under high glucose conditions, which can be alleviated by metformin treatment. This novel multi‐organoid system can recapitulate human‐relevant liver‐islet axis under both physiological and pathological conditions, providing a unique platform for future T2DM research and drug development.
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Erschienen in
-
Microengineered Multi‐Organoid System from hiPSCs to Recapitulate Human Liver‐Islet Axis in Normal and Type 2 Diabetes ; day:23 ; month:12 ; year:2021 ; extent:13
Advanced science ; (23.12.2021) (gesamt 13)
- Urheber
-
Tao, Tingting
Deng, Pengwei
Wang, Yaqing
Zhang, Xu
Guo, Yaqiong
Chen, Wenwen
Qin, Jianhua
- DOI
-
10.1002/advs.202103495
- URN
-
urn:nbn:de:101:1-2021122414145479485904
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
15.08.2025, 07:27 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Tao, Tingting
- Deng, Pengwei
- Wang, Yaqing
- Zhang, Xu
- Guo, Yaqiong
- Chen, Wenwen
- Qin, Jianhua
Ähnliche Objekte (12)
Simulating the Last Glacial Maximum and abrupt glacial climate shifts in a coupled Earth System Model
Evaluating the effects of forward guidance and large-scale asset purchases
A new measure of monetary policy shocks
Fast-food consumption and child body mass index in China: Application of an endogenous switching regression model
Additive and multiplicative hazards modeling for recurrent event data analysis
Residual-Based a Posteriori Error Estimation for Immersed Finite Element Methods
Methamphetamine intoxication in a dog: case report
Prevalence of hyponatremia among older inpatients in a general hospital
Real-world video superresolution enhancement method based on the adaptive down-sampling model
A model-data comparison of the Last Glacial Maximum surface temperature changes
Establishment of genome editing methods and mechanistic dissection of the role of fallen angel during sarcomere maturation in Drosophila
Online Power Control and Optimization for Energy Harvesting Communication System Based on State of Charge
Simulating the Last Glacial Maximum and abrupt glacial climate shifts in a coupled Earth System Model
Evaluating the effects of forward guidance and large-scale asset purchases
A new measure of monetary policy shocks
Fast-food consumption and child body mass index in China: Application of an endogenous switching regression model
Additive and multiplicative hazards modeling for recurrent event data analysis
Residual-Based a Posteriori Error Estimation for Immersed Finite Element Methods
Methamphetamine intoxication in a dog: case report
Prevalence of hyponatremia among older inpatients in a general hospital
Real-world video superresolution enhancement method based on the adaptive down-sampling model
A model-data comparison of the Last Glacial Maximum surface temperature changes
Establishment of genome editing methods and mechanistic dissection of the role of fallen angel during sarcomere maturation in Drosophila
Online Power Control and Optimization for Energy Harvesting Communication System Based on State of Charge
Simulating the Last Glacial Maximum and abrupt glacial climate shifts in a coupled Earth System Model
Evaluating the effects of forward guidance and large-scale asset purchases
A new measure of monetary policy shocks
Fast-food consumption and child body mass index in China: Application of an endogenous switching regression model
Additive and multiplicative hazards modeling for recurrent event data analysis
Residual-Based a Posteriori Error Estimation for Immersed Finite Element Methods
Methamphetamine intoxication in a dog: case report
Prevalence of hyponatremia among older inpatients in a general hospital
Real-world video superresolution enhancement method based on the adaptive down-sampling model
A model-data comparison of the Last Glacial Maximum surface temperature changes
Establishment of genome editing methods and mechanistic dissection of the role of fallen angel during sarcomere maturation in Drosophila