Methylation-specific PCR: four steps in primer design
Abstract: Methylation-specific PCR (MSP) is still the method of choice for a single gene methylation study. The proper design of the primer pairs is a prerequisite for obtaining reliable PCR results. Despite numerous protocols describing the rules for MSP primer design, none of them provide a comprehensive approach to the problem. Our aim was to depict a workflow for the primer design that is concise and easy to follow. In order to achieve this goal, adequate tools for promoter sequence retrieval, MSP primer design and subsequent in silico analysis are presented and discussed. Furthermore, a few instructive examples regarding a good versus a poor primer design are provided. Finally, primer design is demonstrated according to the proposed workflow. This article aims to provide researchers, interested in a single gene methylation studies, with useful information regarding successful primer design.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Methylation-specific PCR: four steps in primer design ; volume:9 ; number:12 ; year:2014 ; pages:1127-1139 ; extent:13
Open life sciences ; 9, Heft 12 (2014), 1127-1139 (gesamt 13)
- Creator
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Davidović, Radoslav
Božović, Ana
Mandušić, Vesna
Krajnović, Milena
- DOI
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10.2478/s11535-014-0324-z
- URN
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urn:nbn:de:101:1-2409211608019.932121553162
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
- 15.08.2025, 7:33 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Davidović, Radoslav
- Božović, Ana
- Mandušić, Vesna
- Krajnović, Milena
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