First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia

Abstract: The impact of in vitro isolation and molecular characterisation of Neospora caninum as well as sequence analyses was studied. The brain homogenate of a naturally Neospora-infected dairy cow (positive in ELISA and Western blot) was intraperitoneally inoculated into Mongolian gerbils (Meriones unguiculatus). The brain of gerbils on day 60 post-inoculation was homogenized, and, after trypsin-digestion, cultured on Vero cells. Neospora-like tachyzoites were first observed after 77 days of cultivation. The parasite was confirmed by polymerase chain reaction (PCR) using Neospora-specific primers Np21 and Np6. The PCR product of the first Slovak isolate (NC-SKB1) was subsequently sequenced and published in GenBank under accession number GU300774. Sequencing and BLAST search identified the isolate as N. caninum.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: First in vitro isolation of Neospora caninum from a naturally infected adult dairy cow in Slovakia ; volume:56 ; number:2 ; year:2011 ; pages:111-115 ; extent:5 ; Acta parasitologica ; 56, Heft 2 (2011), 111-115 (gesamt 5)

Contributors, Places and Time

• Creator: Reiterová, Katarína ; Špilovská, Silvia ; Čobádiová, Andrea ; Mucha, Rastislav

Further information

• DOI: 10.2478/s11686-011-0019-9
• URN: urn:nbn:de:101:1-2405081634591.488915134199
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 14.08.2025, 10:58 AM CEST