VarID2 quantifies gene expression noise dynamics and unveils functional heterogeneity of ageing hematopoietic stem cells
Abstract: Variability of gene expression due to stochasticity of transcription or variation of extrinsic signals, termed biological noise, is a potential driving force of cellular differentiation. Utilizing single-cell RNA-sequencing, we develop VarID2 for the quantification of biological noise at single-cell resolution. VarID2 reveals enhanced nuclear versus cytoplasmic noise, and distinct regulatory modes stratified by correlation between noise, expression, and chromatin accessibility. Noise levels are minimal in murine hematopoietic stem cells (HSCs) and increase during differentiation and ageing. Differential noise identifies myeloid-biased Dlk1+ long-term HSCs in aged mice with enhanced quiescence and self-renewal capacity. VarID2 reveals noise dynamics invisible to conventional single-cell transcriptome analysis
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Anmerkungen
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Genome biology. - 24 (2023) , 148, ISSN: 1474-760X
- Ereignis
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Veröffentlichung
- (wo)
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Freiburg
- (wer)
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Universität
- (wann)
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2024
- Urheber
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Rosales-Alvarez, Reyna Edith
Rettkowski, Jasmin
Herman, Josip S.
Dumbović, Gabrijela
Cabezas Wallscheid, Nina
Grün, Dominic
- DOI
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10.1186/s13059-023-02974-1
- URN
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urn:nbn:de:bsz:25-freidok-2540156
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
- 14.08.2025, 10:45 MESZ
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Beteiligte
- Rosales-Alvarez, Reyna Edith
- Rettkowski, Jasmin
- Herman, Josip S.
- Dumbović, Gabrijela
- Cabezas Wallscheid, Nina
- Grün, Dominic
- Universität
Entstanden
- 2024
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