Lineage tracing identifies in vitro microglia‐to‐neuron conversion by NeuroD1 expression
Abstract: Neuronal regeneration to replenish lost neurons after injury is critical for brain repair. Microglia, brain-resident macrophages that have the propensity to accumulate at the site of injury, can be a potential source for replenishing lost neurons through fate conversion into neurons, induced by forced expression of neuronal lineage-specific transcription factors. However, it has not been strictly demonstrated that microglia, rather than central nervous system-associated macrophages, such as meningeal macrophages, convert into neurons. Here, we show that NeuroD1-transduced microglia can be successfully converted into neurons in vitro using lineage-mapping strategies. We also found that a chemical cocktail treatment further promoted NeuroD1-induced microglia-to-neuron conversion. NeuroD1 with loss-of-function mutation, on the other hand, failed to induce the neuronal conversion. Our results indicate that microglia are indeed reprogrammed into neurons by NeuroD1 with neurogenic transcriptional activity
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
Genes to cells. - 28, 7 (2023) , 526-534, ISSN: 1365-2443
- Klassifikation
-
Medizin, Gesundheit
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- Urheber
-
Irie, Takashi
Matsuda‐Ito, Kanae
Matsuda, Taito
Masuda, Takahiro
Prinz, Marco
Isobe, Noriko
Nakashima, Kinichi
- DOI
-
10.1111/gtc.13033
- URN
-
urn:nbn:de:bsz:25-freidok-2359993
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
25.03.2025, 13:50 MEZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Irie, Takashi
- Matsuda‐Ito, Kanae
- Matsuda, Taito
- Masuda, Takahiro
- Prinz, Marco
- Isobe, Noriko
- Nakashima, Kinichi
- Universität
Entstanden
- 2023
Ähnliche Objekte (12)
Microglia: same same, but different
Morphometrie und Ontogenese von Interneuronen des Gyrus praecentralis peri- und postnataler Entwicklungsstufen des Menschen
Life and death of microglia: Mechanisms governing microglial states and fates
The roles of microglia in viral encephalitis: from sensome to therapeutic targeting
Microglia heterogeneity in the single-cell era
Somatic mosaicism in microglia - a model for neurodegeneration in histiocytosis and implications for treatment
Microglia regulate central nervous system myelin growth and integrity
Author correction: Microglia regulate central nervous system myelin growth and integrity
GPCRomics of homeostatic and disease-associated human microglia
The impact of host gut microbiota on microglia in Alzheimer’s disease : : a study in the 5xFAD transgenic murine model
Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease
Deciphering the heterogeneity of myeloid cells during neuroinflammation in the single‐cell era
Microglia: same same, but different
Morphometrie und Ontogenese von Interneuronen des Gyrus praecentralis peri- und postnataler Entwicklungsstufen des Menschen
Life and death of microglia: Mechanisms governing microglial states and fates
The roles of microglia in viral encephalitis: from sensome to therapeutic targeting
Microglia heterogeneity in the single-cell era
Somatic mosaicism in microglia - a model for neurodegeneration in histiocytosis and implications for treatment
Microglia regulate central nervous system myelin growth and integrity
Author correction: Microglia regulate central nervous system myelin growth and integrity
GPCRomics of homeostatic and disease-associated human microglia
The impact of host gut microbiota on microglia in Alzheimer’s disease : : a study in the 5xFAD transgenic murine model
Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease
Deciphering the heterogeneity of myeloid cells during neuroinflammation in the single‐cell era
Microglia: same same, but different
Morphometrie und Ontogenese von Interneuronen des Gyrus praecentralis peri- und postnataler Entwicklungsstufen des Menschen
Life and death of microglia: Mechanisms governing microglial states and fates
The roles of microglia in viral encephalitis: from sensome to therapeutic targeting
Microglia heterogeneity in the single-cell era
Somatic mosaicism in microglia - a model for neurodegeneration in histiocytosis and implications for treatment
Microglia regulate central nervous system myelin growth and integrity
Author correction: Microglia regulate central nervous system myelin growth and integrity
GPCRomics of homeostatic and disease-associated human microglia
The impact of host gut microbiota on microglia in Alzheimer’s disease : : a study in the 5xFAD transgenic murine model
Single cell RNA sequencing of human microglia uncovers a subset associated with Alzheimer's disease