Histone variant H2A.Z regulates zygotic genome activation
Abstract: During embryogenesis, the genome shifts from transcriptionally quiescent to extensively active in a process known as Zygotic Genome Activation (ZGA). In Drosophila, the pioneer factor Zelda is known to be essential for the progression of development; still, it regulates the activation of only a small subset of genes at ZGA. However, thousands of genes do not require Zelda, suggesting that other mechanisms exist. By conducting GRO-seq, HiC and ChIP-seq in Drosophila embryos, we demonstrate that up to 65% of zygotically activated genes are enriched for the histone variant H2A.Z. H2A.Z enrichment precedes ZGA and RNA Polymerase II loading onto chromatin. In vivo knockdown of maternally contributed Domino, a histone chaperone and ATPase, reduces H2A.Z deposition at transcription start sites, causes global downregulation of housekeeping genes at ZGA, and compromises the establishment of the 3D chromatin structure. We infer that H2A.Z is essential for the de novo establishment of transcriptional programs during ZGA via chromatin reorganization
- Standort
-
Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
-
Online-Ressource
- Sprache
-
Englisch
- Anmerkungen
-
ISSN: 2041-1723
- Klassifikation
-
Biowissenschaften, Biologie
- Ereignis
-
Veröffentlichung
- (wo)
-
Freiburg
- (wer)
-
Universität
- (wann)
-
2023
- DOI
-
10.1038/s41467-021-27125-7
- URN
-
urn:nbn:de:bsz:25-freidok-2369122
- Rechteinformation
-
Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
-
25.03.2025, 13:50 MEZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Universität
Entstanden
- 2023
Ähnliche Objekte (12)
Histone methyltransferase SETDB1 regulates fetal hematopoiesis
Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding
Role of histone variant H3.3 in neurogenesis and gliomagenesis
The role of histone variant H2A.J in radiation dermatitis
Role of histone variant H3.3 in neurogenesis and gliomagenesis
Histone H3 Variant Regulates RNA Polymerase II Transcription Termination and Dual Strand Transcription of siRNA Loci in Trypanosoma brucei
Elucidating the role of histone variant H2A.J following ionizing radiation
The histone demethylase JMJD2B epigenetically regulates endothelial to mesenchymal transition
New insights into the histone variant H2A.Z incorporation pathway in \(Trypanosoma\) \(brucei\)
Unraveling deposition mechanism and function of a new histone H3 variant H3.Y
Competition for DNA binding between histones and transcription factors regulates the timing of zygotic genome activation in zebrafish embryos
Identifying genome-wide transcription units from histone modifications using EPIGENE
Histone methyltransferase SETDB1 regulates fetal hematopoiesis
Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding
Role of histone variant H3.3 in neurogenesis and gliomagenesis
The role of histone variant H2A.J in radiation dermatitis
Role of histone variant H3.3 in neurogenesis and gliomagenesis
Histone H3 Variant Regulates RNA Polymerase II Transcription Termination and Dual Strand Transcription of siRNA Loci in Trypanosoma brucei
Elucidating the role of histone variant H2A.J following ionizing radiation
The histone demethylase JMJD2B epigenetically regulates endothelial to mesenchymal transition
New insights into the histone variant H2A.Z incorporation pathway in \(Trypanosoma\) \(brucei\)
Unraveling deposition mechanism and function of a new histone H3 variant H3.Y
Competition for DNA binding between histones and transcription factors regulates the timing of zygotic genome activation in zebrafish embryos
Identifying genome-wide transcription units from histone modifications using EPIGENE
Histone methyltransferase SETDB1 regulates fetal hematopoiesis
Histone H2A C-terminus regulates chromatin dynamics, remodeling, and histone H1 binding
Role of histone variant H3.3 in neurogenesis and gliomagenesis
The role of histone variant H2A.J in radiation dermatitis
Role of histone variant H3.3 in neurogenesis and gliomagenesis
Histone H3 Variant Regulates RNA Polymerase II Transcription Termination and Dual Strand Transcription of siRNA Loci in Trypanosoma brucei
Elucidating the role of histone variant H2A.J following ionizing radiation
The histone demethylase JMJD2B epigenetically regulates endothelial to mesenchymal transition
New insights into the histone variant H2A.Z incorporation pathway in \(Trypanosoma\) \(brucei\)
Unraveling deposition mechanism and function of a new histone H3 variant H3.Y
Competition for DNA binding between histones and transcription factors regulates the timing of zygotic genome activation in zebrafish embryos