MASTL promotes cell contractility and motility through kinase-independent signaling
Abstract: Microtubule-associated serine/threonine-protein kinase-like (MASTL) is a mitosis-accelerating kinase with emerging roles in cancer progression. However, possible cell cycle–independent mechanisms behind its oncogenicity remain ambiguous. Here, we identify MASTL as an activator of cell contractility and MRTF-A/SRF (myocardin-related transcription factor A/serum response factor) signaling. Depletion of MASTL increased cell spreading while reducing contractile actin stress fibers in normal and breast cancer cells and strongly impairing breast cancer cell motility and invasion. Transcriptome and proteome profiling revealed MASTL-regulated genes implicated in cell movement and actomyosin contraction, including Rho guanine nucleotide exchange factor 2 (GEF-H1, ARHGEF2) and MRTF-A target genes tropomyosin 4.2 (TPM4), vinculin (VCL), and nonmuscle myosin IIB (NM-2B, MYH10). Mechanistically, MASTL associated with MRTF-A and increased its nuclear retention and transcriptional activity. Importantly, MASTL kinase activity was not required for regulation of cell spreading or MRTF-A/SRF transcriptional activity. Taken together, we present a previously unknown kinase-independent role for MASTL as a regulator of cell adhesion, contractility, and MRTF-A/SRF activity
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Notes
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Journal of cell biology. - 219, 6 (2020) , e201906204, ISSN: 1540-8140
- Event
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Veröffentlichung
- (where)
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Freiburg
- (who)
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Universität
- (when)
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2020
- Creator
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Taskinen, Maria Emilia
Närvä, Elisa
Conway, James R. W.
Hinojosa, Laura Soto
Lilla, Sergio
Mai, Anja
De Franceschi, Nicola
Elo, Laura L.
Grosse, Robert
Zanivan, Sara
Norman, Jim C.
Ivaska, Johanna
- DOI
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10.1083/jcb.201906204
- URN
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urn:nbn:de:bsz:25-freidok-1670811
- Rights
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Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
- 14.08.2025, 10:54 AM CEST
Data provider
Deutsche Nationalbibliothek. If you have any questions about the object, please contact the data provider.
Associated
- Taskinen, Maria Emilia
- Närvä, Elisa
- Conway, James R. W.
- Hinojosa, Laura Soto
- Lilla, Sergio
- Mai, Anja
- De Franceschi, Nicola
- Elo, Laura L.
- Grosse, Robert
- Zanivan, Sara
- Norman, Jim C.
- Ivaska, Johanna
- Universität
Time of origin
- 2020
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Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility
Initial contact guidance during cell spreading is contractility-independent
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Detection of drug effects on gastric emptying and contractility using a wireless motility capsule
P38 MAP Kinase inhibition promotes primary tumour growth via VEGF independent mechanism
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Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility
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BCKDH kinase promotes hepatic gluconeogenesis independent of BCKDHA
Protein kinase C and human uterine contractility
Praxillin and focal adhesion kinase regulate cardiac contractility independent of integrin-linked kinase-parvin-PINCH-protein kinase B signaling in zebrafish
Decision tree modeling predicts effects of inhibiting contractility signaling on cell motility
Initial contact guidance during cell spreading is contractility-independent
Frustrated endocytosis controls contractility-independent mechanotransduction at clathrin-coated structures
Detection of drug effects on gastric emptying and contractility using a wireless motility capsule
P38 MAP Kinase inhibition promotes primary tumour growth via VEGF independent mechanism
CapG promoted nasopharyngeal carcinoma cell motility involving Rho motility pathway independent of ROCK
Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility
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