Single cell clonal analysis identifies an AID ‐dependent pathway of plasma cell differentiation
Abstract: Germinal centers (GC) are microstructures where B cells that have been activated by antigen can improve the affinity of their B cell receptors and differentiate into memory B cells (MBCs) or antibody‐secreting plasma cells. Here, we have addressed the role of activation‐induced deaminase (AID), which initiates somatic hypermutation and class switch recombination, in the terminal differentiation of GC B cells. By combining single cell transcriptome and immunoglobulin clonal analysis in a mouse model that traces AID‐experienced cells, we have identified a novel subset of late‐prePB cells (L‐prePB), which shares the strongest clonal relationships with plasmablasts (PBs). Mice lacking AID have various alterations in the size and expression profiles of transcriptional clusters. We find that AID deficiency leads to a reduced proportion of L‐prePB cells and severely impairs transitions between the L‐prePB and the PB subsets. Thus, AID shapes the differentiation fate of GC B cells by enabling PB generation from a prePB state.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Single cell clonal analysis identifies an AID ‐dependent pathway of plasma cell differentiation ; day:07 ; month:10 ; year:2022 ; extent:23
EMBO reports / European Molecular Biology Organization ; (07.10.2022) (gesamt 23)
- Urheber
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Gómez‐Escolar, Carmen
Serrano‐Navarro, Alvaro
Benguria, Alberto
Dopazo, Ana
Sánchez‐Cabo, Fátima
Ramiro, Almudena R.
- DOI
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10.15252/embr.202255000
- URN
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urn:nbn:de:101:1-2022100815010972053103
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:27 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Gómez‐Escolar, Carmen
- Serrano‐Navarro, Alvaro
- Benguria, Alberto
- Dopazo, Ana
- Sánchez‐Cabo, Fátima
- Ramiro, Almudena R.
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