Mouse fetal intestinal organoids: new model to study epithelial maturation from suckling to weaning
Abstract: During the suckling‐to‐weaning transition, the intestinal epithelium matures, allowing digestion of solid food. Transplantation experiments with rodent fetal epithelium into subcutaneous tissue of adult animals suggest that this transition is intrinsically programmed and occurs in the absence of dietary or hormonal signals. Here, we show that organoids derived from mouse primary fetal intestinal epithelial cells express markers of late fetal and neonatal development. In a stable culture medium, these fetal epithelium‐derived organoids lose all markers of neonatal epithelium and start expressing hallmarks of adult epithelium in a time frame that mirrors epithelial maturation in vivo. In vitro postnatal development of the fetal‐derived organoids accelerates by dexamethasone, a drug used to accelerate intestinal maturation in vivo. Together, our data show that organoids derived from fetal epithelium undergo suckling‐to‐weaning transition, that the speed of maturation can be modulated, and that fetal organoids can be used to model the molecular mechanisms of postnatal epithelial maturation.
- Location
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Deutsche Nationalbibliothek Frankfurt am Main
- Extent
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Online-Ressource
- Language
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Englisch
- Bibliographic citation
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Mouse fetal intestinal organoids: new model to study epithelial maturation from suckling to weaning ; volume:20 ; number:2 ; year:2019 ; extent:12
EMBO reports / European Molecular Biology Organization ; 20, Heft 2 (2019) (gesamt 12)
- Creator
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Navis, Marit
Martins Garcia, Tânia
Renes, Ingrid B.
Vermeulen, Jacqueline LM
Meisner, Sander
Wildenberg, Manon E.
van den Brink, Gijs R.
van Elburg, Ruurd M.
Muncan, Vanesa
- DOI
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10.15252/embr.201846221
- URN
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urn:nbn:de:101:1-2022072009092475314084
- Rights
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Last update
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15.08.2025, 7:25 AM CEST
Data provider
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Associated
- Navis, Marit
- Martins Garcia, Tânia
- Renes, Ingrid B.
- Vermeulen, Jacqueline LM
- Meisner, Sander
- Wildenberg, Manon E.
- van den Brink, Gijs R.
- van Elburg, Ruurd M.
- Muncan, Vanesa
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