Electrochemically modulated single-molecule localization microscopy for in vitro imaging cytoskeletal protein structures

Abstract: A new concept of electrochemically modulated single-molecule localization super-resolution imaging is developed. Applications of single-molecule localization super-resolution microscopy have been limited due to insufficient availability of qualified fluorophores with favorable low duty cycles. The key for the new concept is that the “On” state of a redox-active fluorophore with unfavorable high duty cycle could be driven to “Off” state by electrochemical potential modulation and thus become available for single-molecule localization imaging. The new concept was carried out using redox-active cresyl violet with unfavorable high duty cycle as a model fluorophore by synchronizing electrochemical potential scanning with a single-molecule localization microscope. The two cytoskeletal protein structures, the microtubules from porcine brain and the actins from rabbit muscle, were selected as the model target structures for the conceptual imaging in vitro. The super-resolution images of microtubules and actins were obtained from precise single-molecule localizations determined by modulating the On/Off states of single fluorophore molecules on the cytoskeletal proteins via electrochemical potential scanning. Importantly, this method could allow more fluorophores even with unfavorable photophysical properties to become available for a wider and more extensive application of single-molecule localization microscopy.

Object Details

• Location: Deutsche Nationalbibliothek Frankfurt am Main
• Extent: Online-Ressource
• Language: Englisch

References and Relationships

• Bibliographic citation: Electrochemically modulated single-molecule localization microscopy for in vitro imaging cytoskeletal protein structures ; volume:14 ; number:4 ; year:2025 ; pages:459-470 ; extent:12 ; Nanophotonics ; 14, Heft 4 (2025), 459-470 (gesamt 12)

Contributors, Places and Time

• Creator: Lei, Chenghong ; Hu, Dehong

Further information

• DOI: 10.1515/nanoph-2024-0559
• URN: urn:nbn:de:101:1-2502191213496.853866841674
• Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
• Last update: 15.08.2025, 7:26 AM CEST