Digital Microfluidics for Sample Preparation in Low‐Input Proteomics
Abstract: Low‐input proteomics, also referred to as micro‐ or nanoproteomics, has become increasingly popular as it allows one to elucidate molecular processes in rare biological materials. A major prerequisite for the analytics of minute protein amounts, e.g., derived from low cell numbers, down to single cells, is the availability of efficient sample preparation methods. Digital microfluidics (DMF), a technology allowing the handling and manipulation of low liquid volumes, has recently been shown to be a powerful and versatile tool to address the challenges in low‐input proteomics. Here, an overview is provided on recent advances in proteomics sample preparation using DMF. In particular, the capability of DMF to isolate proteomes from cells and small model organisms, and to perform all necessary chemical sample preparation steps, such as protein denaturation and proteolytic digestion on‐chip, are highlighted. Additionally, major prerequisites to making these steps compatible with follow‐up analytical methods such as liquid chromatography‐mass spectrometry will be discussed.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Digital Microfluidics for Sample Preparation in Low‐Input Proteomics ; day:29 ; month:08 ; year:2024 ; extent:16
Small Methods ; (29.08.2024) (gesamt 16)
- Urheber
- DOI
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10.1002/smtd.202400495
- URN
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urn:nbn:de:101:1-2408291428492.527330620876
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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15.08.2025, 07:26 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Steinbach, Max K.
- Leipert, Jan
- Matzanke, Theo
- Tholey, Andreas
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