Squaramate‐Modified Nucleotides and DNA for Specific Cross‐Linking with Lysine‐Containing Peptides and Proteins

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Abstract: Squaramate‐linked 2′‐deoxycytidine 5′‐O‐triphosphate was synthesized and found to be good substrate for KOD XL DNA polymerase in primer extension or PCR synthesis of modified DNA. The resulting squaramate‐linked DNA reacts with primary amines to form a stable diamide linkage. This reaction was used for bioconjugations of DNA with Cy5 and Lys‐containing peptides. Squaramate‐linked DNA formed covalent cross‐links with histone proteins. This reactive nucleotide has potential for other bioconjugations of nucleic acids with amines, peptides or proteins without need of any external reagent.

Location: Deutsche Nationalbibliothek Frankfurt am Main

Extent: Online-Ressource

Language: Englisch

Bibliographic citation: Squaramate‐Modified Nucleotides and DNA for Specific Cross‐Linking with Lysine‐Containing Peptides and Proteins ; volume:131 ; number:38 ; year:2019 ; pages:13479-13482 ; extent:4
Angewandte Chemie ; 131, Heft 38 (2019), 13479-13482 (gesamt 4)

Creator: Ivancová, Ivana
Pohl, Radek
Hubálek, Martin
Hocek, Michal

DOI: 10.1002/ange.201906737

URN: urn:nbn:de:101:1-2022070808212411259288

Rights: Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.

Last update: 15.08.2025, 7:32 AM CEST

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Associated

Ivancová, Ivana
Pohl, Radek
Hubálek, Martin
Hocek, Michal

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