Super‐Resolution Detection of DNA Nanostructures Using a Nanopore
Abstract: High‐resolution analysis of biomolecules has brought unprecedented insights into fundamental biological processes and dramatically advanced biosensing. Notwithstanding the ongoing resolution revolution in electron microscopy and optical imaging, only a few methods are presently available for high‐resolution analysis of unlabeled single molecules in their native states. Here, label‐free electrical sensing of structured single molecules with a spatial resolution down to single‐digit nanometers is demonstrated. Using a narrow solid‐state nanopore, the passage of a series of nanostructures attached to a freely translocating DNA molecule is detected, resolving individual nanostructures placed as close as 6 nm apart and with a surface‐to‐surface gap distance of only 2 nm. Such super‐resolution ability is attributed to the nanostructure‐induced enhancement of the electric field at the tip of the nanopore. This work demonstrates a general approach to improving the resolution of single‐molecule nanopore sensing and presents a critical advance towards label‐free, high‐resolution DNA sequence mapping, and digital information storage independent of molecular motors.
- Standort
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Deutsche Nationalbibliothek Frankfurt am Main
- Umfang
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Online-Ressource
- Sprache
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Englisch
- Erschienen in
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Super‐Resolution Detection of DNA Nanostructures Using a Nanopore ; day:12 ; month:02 ; year:2023 ; extent:9
Advanced materials ; (12.02.2023) (gesamt 9)
- Urheber
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Chen, Kaikai
Choudhary, Adnan
Sandler, Sarah E.
Maffeo, Christopher
Ducati, Caterina
Aksimentiev, Aleksei
Keyser, Ulrich F.
- DOI
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10.1002/adma.202207434
- URN
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urn:nbn:de:101:1-2023021314073349262587
- Rechteinformation
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Open Access; Der Zugriff auf das Objekt ist unbeschränkt möglich.
- Letzte Aktualisierung
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14.08.2025, 11:01 MESZ
Datenpartner
Deutsche Nationalbibliothek. Bei Fragen zum Objekt wenden Sie sich bitte an den Datenpartner.
Beteiligte
- Chen, Kaikai
- Choudhary, Adnan
- Sandler, Sarah E.
- Maffeo, Christopher
- Ducati, Caterina
- Aksimentiev, Aleksei
- Keyser, Ulrich F.
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